Does the Site of Blood Collection in Fish Affect Haematological and Blood Biochemical Results?

IF 0.8 4区生物学 Q4 BIOLOGY

Folia Biologica-Krakow Pub Date : 2021-07-13 DOI:10.3409/FB_69-2.07

B. Bojarski, M. Socha, E. Drąg-Kozak, A. Rombel-Bryzek, S. Kapinos, L. Szała, E. Kondera, K. Lugowska, M. Witeska

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引用次数: 5

Abstract

The values of haematological and selected blood plasma biochemical parameters of juvenile common carp (Cyprinus carpio Linnaeus, 1758) were compared between blood samples taken from caudal vein and heart to evaluate the influence of blood sampling body site on the obtained results in two groups of fish of different blood sampling order: I – first by caudal and then by cardiac puncture, II – first by cardiac and then by caudal puncture. The obtained results revealed statistically significant (p<0.05) differences only in group I where red blood cell (RBC) count was higher in caudal vein blood, while haematocrit (Ht) value, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), total protein (TP) concentration, and magnesium (Mg) level were higher in cardiac blood samples. No statistically significant differences occurred in white blood cell (WBC) count, differential leukocyte count or erythrocyte morphology based on stained blood smears. The obtained results showed that blood sampling body site may affect the results of haematological and plasma biochemical analyses.

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鱼类的采血部位会影响血液学和血液生化结果吗？

将幼年鲤鱼（Cyprinus carpio Linnaeus，1758）的血液学和选定的血浆生化参数值与从尾静脉和心脏采集的血液样本进行比较，以评估不同采血顺序的两组鱼的采血部位对所获得结果的影响：I–先通过尾静脉，然后通过心脏穿刺，II–首先通过心脏穿刺，然后通过尾部穿刺。所获得的结果显示，只有在I组中，尾静脉血液中的红细胞（RBC）计数较高，而心脏血液样本中的红血球压积（Ht）值、平均红细胞体积（MCV）、平均红血球血红蛋白（MCH）、总蛋白（TP）浓度和镁（Mg）水平较高，差异具有统计学意义（p<0.05）。基于染色血涂片的白细胞（WBC）计数、差异白细胞计数或红细胞形态没有统计学上的显著差异。所得结果表明，采血部位可能影响血液学和血浆生化分析的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Biologica-Krakow 医学-生物学

CiteScore

1.10

自引率

14.30%

发文量

审稿时长

>12 weeks

期刊介绍： Folia Biologica (Kraków) is an international online open access journal accepting original scientific articles on various aspects of zoology: phylogeny, genetics, chromosomal studies, ecology, biogeography, experimental zoology and ultrastructural studies. The language of publication is English, articles are assembled in four issues per year.