Molecular analysis of human adenoviral keratoconjunctivitis cases: Results of a 2-year survey

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL

Marmara Medical Journal Pub Date : 2023-01-31 DOI:10.5472/marumj.1244369

Ayfer Guner, Rabia Can Sarınoğlu, F. Aydin, S. Akkaya Turhan, M. Kuşkucu, A. E. Toker, Aysegul Karahasan Yagci

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Abstract

Objective: This study aimed to determine the adenovirus genotypes and their epidemiological features between January 2018 and November 2019, in Istanbul, Turkey. Material and Methods: Conjunctival swab samples were obtained from patients who were clinically diagnosed with keratoconjunctivitis. Samples were screened with an Adeno Detector kit (Rapid Pathogen Screening, RPS Inc., South Williamsport, PA). Nucleic acid extraction and amplification were performed with the ADENOVIRUS ELITe MGB® kit in the ELITe In Genius instrument (Elitech Group, Torino, Italy). For subtyping of the strains, sequencing primers targeted the ‘Hypervariable Region 7’ (HVR-7) of the hexon gene were used. DNA sequence analysis (n:72) was performed with ABI PRISM® 3100 Genetic Analyzer (Applied Biosystems, USA), and subtyping was done by BLAST analysis. Results: The median viral load in the samples (n: 77) was 7 log10 copies/mL (IQR: 4.5-7.4 log10 copies/mL). The clinical finding score was found to be significantly higher in the high viral load group (Adenovirus DNA≥6 Log 10 copies/mL) than in the low viral load group (Adenovirus DNA

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人类腺病毒性角结膜炎病例的分子分析：2年调查结果

目的:了解2018年1月至2019年11月土耳其伊斯坦布尔地区腺病毒基因型及其流行病学特征。材料和方法:从临床诊断为角膜结膜炎的患者中获得结膜拭子样本。样品用腺病毒检测试剂盒(快速病原体筛选，RPS公司，South Williamsport, PA)筛选。在ELITe in Genius仪器(ELITe Group, Torino, Italy)中使用腺病毒ELITe MGB®试剂盒进行核酸提取和扩增。采用针对六邻体基因“高变区7”(HVR-7)的测序引物进行分型。DNA序列分析(n:72)使用ABI PRISM®3100遗传分析仪(Applied Biosystems, USA)进行，并通过BLAST分析进行分型。结果:样本(n: 77)的中位病毒载量为7 log10拷贝/mL (IQR: 4.5-7.4 log10拷贝/mL)。高病毒载量组(腺病毒DNA≥6 Log 10拷贝/mL)的临床表现评分明显高于低病毒载量组(腺病毒DNA)

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来源期刊

Marmara Medical Journal MEDICINE, GENERAL & INTERNAL-

CiteScore

0.30

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0.00%

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期刊介绍： Marmara Medical Journal, Marmara Üniversitesi Tıp Fakültesi tarafından yılda üç kere yayımlanan multidisipliner bir dergidir. Bu dergide tıbbın tüm alanlarına ait orijinal araştırma makaleleri, olgu sunumları ve derlemeler İngilizce veya Türkçe olarak yer alır.