Indigenous Lactic Acid Bacteria from Halloumi Cheese as a Probiotics Candidate of Indonesian Origin

Q4 Medicine
D. Ratna, M.M. Evita, E. S. Rahayu, M. N. Cahyanto, R. Wikandari, T. Utami
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引用次数: 2

Abstract

This study aimed to isolate lactic acid bacteria from Halloumi cheese produced by Mazaarat Artisan cheese Indonesia, to evaluate its potential as a probiotics, and to identify it based on molecular characteristics. The lactic acid bacteria were isolated from fresh milk, curd, and the Halloumi cheese and their morphology and physiology were identified. Screening of the lactic acid bacteria isolates for probiotics candidates was mainly based on the resistance of simulated gastric juice, bile salts, antibacterial activities against pathogens, and the adhesion properties by hydrophobicity test. The selected lactic acid bacteria strains as probiotics candidates were then identified molecularly using 16S ribosomal RNA. From this study, 13 lactic acid bacteria isolates were obtained. Among the 13 isolates, K3 isolate was the most potent as a probiotics candidate. This isolate had high tolerance for simulated gastric juice and bile salts, good ability to protect the gut against pathogenic bacteria since it has antibacterial activities and had ability to adhere to human epithelial cells. Based on molecular identification, this probiotics candidate was identified as Lactobacillus plantarum.
印尼产Halloumi奶酪的乳酸菌研究
本研究旨在从印度尼西亚Mazaarat Artisan奶酪公司生产的Halloumi奶酪中分离乳酸菌,评估其作为益生菌的潜力,并根据分子特征对其进行鉴定。从鲜奶、凝乳和哈洛米干酪中分离出乳酸菌,并对其形态和生理进行了鉴定。乳酸菌分离株对益生菌候选菌株的筛选主要基于模拟胃液、胆汁盐的耐药性、对病原体的抗菌活性以及疏水性测试的粘附特性。然后使用16S核糖体RNA对所选乳酸菌菌株作为益生菌候选菌株进行分子鉴定。本研究共获得13株乳酸菌。在13个分离株中,K3分离株是最有效的益生菌候选株。该分离物对模拟胃液和胆汁盐具有高耐受性,由于具有抗菌活性并能够粘附于人类上皮细胞,因此具有良好的保护肠道免受致病菌感染的能力。基于分子鉴定,该候选益生菌被鉴定为植物乳杆菌。
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来源期刊
International Journal of Probiotics and Prebiotics
International Journal of Probiotics and Prebiotics Nursing-Nutrition and Dietetics
CiteScore
1.20
自引率
0.00%
发文量
7
期刊介绍: The International journal of Probiotics & Prebiotics publishes on online only in an open access format. This is a broad based international, interdisciplinary peer reviewed scientific journal for critical evaluation of research on prebiotics, probiotics and synbiotics. The major goal of this journal is to provide unbiased scientific data to students, researchers, healthcare providers, and the decision makers in the nutraceutical industry to help make informed choices about prebiotics, probiotics and synbiotics. To this end, the journal will publish original research articles and two types of review articles. First, we will publish a review of preclinical research data coming largely from animal, cell culture and other experimental models. Such data will provide basis for future product development and/or human research initiatives. Second, we will publish a critical evaluation of current human experimental data to help deliver products with medically proven use.
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