EFFECT OF CHITOSAN AND META-TOPOLIN IN MICROPROPAGATION OF VACCINIUM CORYMBOSUM

IF 0.8 Q4 MATERIALS SCIENCE, BIOMATERIALS

Progress on Chemistry and Application of Chitin and its Derivatives Pub Date : 2022-09-30 DOI:10.15259/pcacd.27.011

M. Krupa-Małkiewicz, Agata Jurkiewicz, I. Ochmian, Monika Figiel-Kroczyńska

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引用次数: 1

Abstract

The culture medium is often optimised to improve growth and morphogenesis in vitro. The efficiency of chitosan (CH) and meta-topolin (mT) on in vitro growth of blueberries (Vaccinium corymbosum) cv Liberty was investigated. The explants were grown on Woody Plant Medium (WPM) supplemented with mT at a concentration of 0.2, 0.4, and 0.6 mg l-1 and WPM with chitosan with a molecular weight 3.33 kDa at 10, 20, and 40 ppm. WPM with the addition of 0.1 mg l-1 zeatin was used as a control. Among the tested combinations, the maximum mean shoot length (23.81 cm), the number of new shoots (1.34), fresh mass (42.29 g), and dry mass (14.24 g) were recorded on the control medium. The addition of lower concentrations of chitosan (10 and 20 ppm) to the WPM medium decreased shoot length (23%-24.5% of control), fresh mass (30%-40% of control), and dry mass (20%-29% of control). However, blueberries treated with 40 ppm chitosan had similar shoot lengths, but darker (112% of control) and greener (139% of control) leaves compared with the control. In contrast, mT had a negative effect on the studied traits of Liberty blueberries, regardless of the concentration.

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壳聚糖和间topolin对球栓苗微繁的影响

培养基经常被优化以提高体外生长和形态发生。研究了壳聚糖(CH)和间topolin (mT)对蓝莓(Vaccinium corymbosum)体外生长的影响。分别在浓度为0.2、0.4和0.6 mg l-1的木本植物培养基(Woody Plant Medium, WPM)和分子量为3.33 kDa的壳聚糖(chitosan)培养基(10、20和40 ppm)上生长。WPM添加0.1 mg l-1玉米素作为对照。试验组合中，对照培养基的最大平均芽长为23.81 cm，新芽数为1.34个，鲜质量为42.29 g，干质量为14.24 g。在WPM培养基中添加较低浓度的壳聚糖(10和20 ppm)，使芽长(23% ~ 24.5%)、鲜质量(30% ~ 40%)和干质量(20% ~ 29%)减少。然而，与对照相比，经40 ppm壳聚糖处理的蓝莓的茎长相似，但叶片颜色更深(占对照的112%)，颜色更绿(占对照的139%)。相比之下，mT对自由蓝莓的研究性状有负影响，无论浓度如何。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Progress on Chemistry and Application of Chitin and its Derivatives MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

1.10

自引率

16.70%

发文量

期刊介绍： Progress in the Chemistry and Application of Chitin and its Derivatives is an annual journal focused on all aspects of production, modification, enzymology and application of chitin and its many derivatives, including chitosan. The journal publishes full-length papers as well as invited reviews. To be considered, papers must present original research that has not been published or accepted for publication elsewhere. The language of the journal will be English.