Changes in PR2 and PAL Patterns in Barley Challenged with Leaf Stripe (Pyrenophora graminea) and Powdery Mildew (Blumeria graminis) Diseases

Acta Phytopathologica Et Entomologica Hungarica Pub Date : 2019-06-27 DOI:10.1556/038.54.2019.005

A. Al-Daoude, H. Alek, M. Jawhar, E. Al-shehadah, A. Shoiab, M. Arabi

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引用次数: 0

Abstract

The seed-borne (Pyrenophora graminea; Pg) and foliar (Blumeria graminis; Bg) are two economically important fungal pathogens of barley worldwide. Barley plant resistance genes, as the pathogenesis related proteins play an important role in defense mechanisms. This study aimed to monitor the expression of PR2 and PAL pathogenesis related genes during compatible/incompatible barley interaction with Pg and Bg at different time points of disease development using the Quantitative Real-time PCR technique (qRT-PCR). Comparison of data showed that PR2 and PAL were significantly over expressed in infected resistant and susceptible plants as against their lower expression in controls,. Upregulation of these defense-related genes during Pg and Bg infections was companied with a slow development of disease symptoms at the time course in the resistant genotype. qRT-PCR analysis revealed higher gene expression in resistant barley plants inoculated with Pg as compared with Bg, with a maximum expression for PR2 (13.8 and 5.06-fold) and PAL (14.8 and 4.51-fold) respectively, at the latest stage of each disease development. It was also noteworthy that PR2 and PAL genes, had higher constitutive expression and faster induction for the both pathogens in the resistant genotype as compared with the susceptible one. Obtained results suggest that both genes, PR2 and PAL, positively regulate Pg- and Bg-resistance in barley plants during disease progress. These expression patterns can provide useful insights to better understanding of the barley–fungus interactions with different fungal lifestyles.

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大麦在叶条病和白粉病胁迫下PR2和PAL模式的变化

种子传播的（Pyrenophora graminea；Pg）和叶面传播的（Blumeria graminis；Bg）是世界范围内大麦的两种经济上重要的真菌病原体。大麦植物抗性基因作为发病机制中的相关蛋白，在防御机制中发挥着重要作用。本研究旨在使用实时定量PCR技术（qRT-PCR）监测在疾病发展的不同时间点，大麦与Pg和Bg的相容/不相容相互作用过程中PR2和PAL发病机制相关基因的表达。数据比较显示，PR2和PAL在受感染的抗性和易感植物中显著过表达，而在对照中表达较低，。在Pg和Bg感染期间，这些防御相关基因的上调伴随着抗性基因型在病程中疾病症状的缓慢发展。qRT-PCR分析显示，与Bg相比，接种Pg的抗性大麦植株的基因表达更高，在每种疾病发展的最新阶段，PR2和PAL的最大表达分别为13.8和5.06倍和14.8和4.51倍。值得注意的是，与易感基因型相比，PR2和PAL基因在抗性基因型中对这两种病原体具有更高的组成型表达和更快的诱导。研究结果表明，PR2和PAL两个基因在病害发生过程中都对大麦植株的Pg和Bg抗性有正向调控作用。这些表达模式可以为更好地理解大麦-真菌与不同真菌生活方式的相互作用提供有用的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Phytopathologica Et Entomologica Hungarica Agricultural and Biological Sciences-Plant Science

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The journal publishes papers on the infectious diseases of plants, damages caused by insects and deals with the basic aspects of chemical and biological protection. Within its field of interest additional topics such as resistance against plant diseases, and physiological, biochemical and molecular questions of plant resistance and susceptibility are also discussed. Publishes book reviews and advertisements.