Assessment of vaginal microbiota and urine seeding on flora in HIV-infected women, depending on the presence or absence of pregravid preparation

Abstract

The purpose of the study: to analyze the microbiota of the vagina and urine culture in HIV-infected women, depending on the absence or presence of pre-pregnancy preparation with and without a partner.Materials and methods. 90 women of reproductive age with stage 3 and 4A of HIV infection participated. Vaginal fluid and urine were inoculated on artificial nutrient media, identification using MALDI ToF mass spectrometry. The Kraskel-Wallis criterion, Mann-Whitney criterion and Simpson index were used to compare the groups.Results and their discussion. It was found out that in women without pregravidar preparation, the total bacterial mass of lactobacilli is significantly lower (p=0.004), the total number of lactobacilli is lower (p=0.005); the bacterial mass of staphylococci and streptococci is greater (p=0.009); the total number of microorganisms seeded is higher (p=0.004) against the background of increased viral load (p=0.001); Enteroccocus often acts as the cause of asymptomatic bacteriuria faecalis with a viral load above the detection threshold (p=0.009). Thus, we show that the lack of pre-pregnancy preparation in HIV-infected women is associated with vaginal mesocenosis and the development of asymptomatic bacteriuria.