Analysis of glucocorticoid receptor gene polymorphisms in kidney recipients with post-transplant diabetes

IF 0.2 Q4 UROLOGY & NEPHROLOGY

Journal of Renal Injury Prevention Pub Date : 2022-04-24 DOI:10.34172/jrip.2022.32052

J. Etemadi, S. Abediazar, T. Majidi, S. Zununi Vahed, R. Motavalli, Bahram Niknafs, Mohamadreza Jafari Nakhjavani

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引用次数: 1

Abstract

Introduction: Post-transplant diabetes mellitus (PTDM) is a severe and common metabolic problem after transplantation. Glucocorticoid receptor (GR) is encoded by the NR3C1 gene and it seems that polymorphisms in this gene lead to altering insulin sensitivity. Objectives: This study aimed to evaluate the frequency of four common polymorphisms in the NR3C1 gene of renal recipients with and without PTDM. Patients and Methods: Blood samples were collected from 32 PTDM and 59 non-diabetic renal-transplanted patients. After DNA extraction, DNA fragments were amplified and directly sequenced using specific primers. Data analysis was performed with SPSS 22.0 software. Results: There was no significant correlation between diabetes incidence and the four investigated polymorphisms of the GR gene. Nevertheless, diabetic patients’ age was higher than non-diabetic patients. Additionally, transplant acute rejection (AR) in diabetic patients was found to be more than non-diabetic patients. Conclusion: Based on gathered information in this research, none of the studied polymorphisms affected the development of PTDM. Further investigations should be conducted in a large sample size.

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肾移植后糖尿病患者糖皮质激素受体基因多态性分析

移植后糖尿病(PTDM)是移植后常见的严重代谢问题。糖皮质激素受体(GR)由NR3C1基因编码，该基因的多态性似乎会导致胰岛素敏感性的改变。目的:本研究旨在评估患有和不患有PTDM的肾受体NR3C1基因四种常见多态性的频率。患者和方法:采集32例PTDM和59例非糖尿病肾移植患者的血液样本。提取DNA后，扩增DNA片段，使用特定引物直接测序。数据分析采用SPSS 22.0软件。结果:GR基因4种多态性与糖尿病发病率无显著相关性。然而，糖尿病患者的年龄高于非糖尿病患者。此外，糖尿病患者的移植急性排斥反应(AR)多于非糖尿病患者。结论:根据本研究收集到的信息，所研究的多态性均不影响PTDM的发展。进一步的调查应在大样本范围内进行。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Renal Injury Prevention UROLOGY & NEPHROLOGY-

CiteScore

1.60

自引率

0.00%

发文量

期刊介绍： The Journal of Renal Injury Prevention (JRIP) is a quarterly peer-reviewed international journal devoted to the promotion of early diagnosis and prevention of renal diseases. It publishes in March, June, September and December of each year. It has pursued this aim through publishing editorials, original research articles, reviews, mini-reviews, commentaries, letters to the editor, hypothesis, case reports, epidemiology and prevention, news and views and renal biopsy teaching point. In this journal, particular emphasis is given to research, both experimental and clinical, aimed at protection/prevention of renal failure and modalities in the treatment of diabetic nephropathy. A further aim of this journal is to emphasize and strengthen the link between renal pathologists/nephropathologists and nephrologists. In addition, JRIP welcomes basic biomedical as well as pharmaceutical scientific research applied to clinical nephrology. Futuristic conceptual hypothesis that integrate various fields of acute kidney injury and renal tubular cell protection are encouraged to be submitted.