Investigation of polymorphisms of exon 1 region of OsHKT1;5 gene in high yielding rice

International journal of genetics and molecular biology Pub Date : 2019-06-30 DOI:10.5897/IJGMB2019.0175

N. Pha, Nguyen Thi My Phung, N. T. Truc, D. Khang, T. Gioi

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引用次数: 2

Abstract

This study was carried out to analyze the polymorphic region of exon 1 of OsHKT1;5 of 22 high yielding rice varieties that are salt tolerant. The result of the phenotypic screening for salinity stress was done using the standard protocol of the International Rice Research Institute which recorded that all rice varieties developed very well under control condition, 85.38% were quite tolerant to salinity treatment of 4‰, 68.18% were moderately tolerant to salinity treatment of 8‰ and 36.36% were moderately tolerant to salinity treatment of 10‰, similar to Pokalli. The result of sequencing exon 1 region of OsHKT1;5 gene recorded five Single Nucleotide Polymorphisms (SNP) markers. Five nucleotide substitutions in coding sequence of OsHKT1;5 were found at the positions: 382, 418, 484, 551 and 994. All five non-synonymous nucleotide substitutions caused changes in amino acids (D51N, P63A, V86I, R107H and H255D). Key words: High yielding rice, OsHKT1;5, single nucleotide polymorphisms (SNP) makers.

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高产水稻OsHKT1;5基因外显子1区多态性的研究

本研究对22个耐盐高产水稻品种OsHKT1;5外显子1的多态性区域进行了分析。采用国际水稻研究所标准方案进行盐胁迫表型筛选，结果显示，在对照条件下，所有水稻品种发育良好，其中4‰的耐盐性为85.38%，8‰的耐盐性为68.18%，10‰的耐盐性为36.36%，与Pokalli相似。OsHKT1;5基因外显子1区测序结果记录5个单核苷酸多态性(Single Nucleotide Polymorphisms, SNP)标记。在OsHKT1编码序列中，382、418、484、551、994位点有5个核苷酸被替换。所有5个非同义核苷酸替换引起氨基酸(D51N, P63A, V86I, R107H和H255D)的变化。关键词:高产水稻，OsHKT1;5，单核苷酸多态性(SNP)制造者

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International journal of genetics and molecular biology

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