Prospective applications of biogenic silver nanoparticles by Moringa oleifera: Apoptosis induction, HT-29 cell cycle arrest, microbial growth inhibition and immune cell activation

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Essam H. Ibrahim, Ali M. Alshehri, H. A. Ghramh, M. Kilany, K. Morsy, H. El-Mekkawy, A. El‐kott, Ramadan Taha, A. Khalofah, M. A. Sayed, I. Aly
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引用次数: 0

Abstract

Objective(s): Moringa oleifera plant is native to several countries and being used in traditional medicine. This study aimed to investigate the effects of M. oleifera leaves acetone extract (MOLAExt), with or without nanoparticles of silver (AgNPs) on cancer cells, bacteria, immune cells and antioxidant activities. Materials and Methods: MOLAExt was used to prepare AgNPs. AgNPs were characterized by UV-Vis-NIR, XRD and SEM. Functional groups, sugars, reactive oxygen species and proteins in the extract were explored and the biological properties were tested. Results: Results demonstrated that AgNPs were of 59 nm in diameter. MOLAExt contained active biomolecules and ROS but no sugars and proteins were detected. MOLAExt and MOLAExt+AgNPs caused a moderate antibacterial action, an increase in apoptotic cells number and in p53 protein expression, and arrested HT-29 colon cancer cell line at G2/M phase, stimulated splenocytes growth and had antioxidant activity.Conclusion: MOLAExt and MOLAExt+AgNP caused apoptosis in cancer cells and stimulated immune cells suggesting that it can be used as anticancer agents.
辣木制备的生物源银纳米颗粒的应用前景:诱导细胞凋亡、阻滞HT-29细胞周期、抑制微生物生长和激活免疫细胞
目的:辣木植物原产于几个国家,被用于传统医学。本研究旨在研究油橄榄叶丙酮提取物(MOLAExt)加或不加银纳米颗粒(AgNPs)对肿瘤细胞、细菌、免疫细胞和抗氧化活性的影响。材料与方法:用MOLAExt制备AgNPs。采用UV-Vis-NIR、XRD和SEM对AgNPs进行了表征。对提取物中的官能团、糖、活性氧和蛋白质进行了研究,并对其生物学特性进行了测试。结果:AgNPs的直径为59 nm。MOLAExt含有活性生物分子和活性氧,但未检测到糖和蛋白质。MOLAExt和MOLAExt+AgNPs具有中等抑菌作用,增加凋亡细胞数量和p53蛋白表达,使HT-29结肠癌细胞系处于G2/M期,刺激脾细胞生长,具有抗氧化活性。结论:MOLAExt及MOLAExt+AgNP可引起肿瘤细胞凋亡,刺激免疫细胞,提示其可作为抗癌药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
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0.00%
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0
审稿时长
12 weeks
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