Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria-Beograd Pub Date : 2021-09-01 DOI:10.2478/acve-2021-0025

Tijana1 Lužajić Božinovski, V. Todorović, I. Milošević, Vladimir Gajdov, B. Prokic, Katarina Nešović, V. Mišković‐Stanković, Danica Marković

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引用次数: 3

Abstract

Abstract A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.

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动物模型中皮下植入银/聚(乙烯醇)和新型银/聚(乙烯醇)/石墨烯水凝胶后软组织再生过程的评价

摘要一种新生产的生物材料必然受到标准的约束，这些标准是在动物模型上进行的。根据国际标准ISO 10993-62007的说明，为了评估皮下植入用于临床的生物材料银/聚乙烯醇（Ag/PVA）和新型银/聚乙烯醇/石墨烯（Ag/PVA/Gr）后软组织再生的可能性，使用了16只大鼠。在移植后7、15、30和60天观察组织学切片。这些水凝胶是通过在聚合物基质中原位电化学合成银纳米颗粒来制备的，这使得能够获得完全安全和生物相容的材料，不含任何额外的有毒化学还原剂。水凝胶的外科植入是根据贝尔格莱德大学兽医学院伦理委员会的许可进行的。免疫组织化学（IHC）研究包括评估血管中平滑肌肌动蛋白的表达（α-SMA）、层粘连蛋白和皮肤结构中I型和III型胶原的表达，以及确定细胞增殖标志物表达（Ki-67）角质形成细胞。以半定量的方式评估结果。数据在IBM SPSS 20统计软件包中进行分析。结论表明，Ag/PVA/Gr可以用作伤口敷料，以增强组织愈合潜力，并在组织中建立更快的整合和更短的滞留时间。

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来源期刊

Acta Veterinaria-Beograd 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

18-36 weeks

期刊介绍： The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.