Accurate Calibration of Optical Tweezers close to a glass surface using interference rings in backscattered light

IF 1.9 4区 物理与天体物理 Q3 OPTICS
N. Westbrook
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引用次数: 0

Abstract

Detecting the retro-reflected trap beam is a convenient way to measure forces applied by optical tweezers on biological systems, leaving free top access to the sample and making it easier to combine with fluorescence and phase contrast imaging. Doing so in the back focal plane of the microscope objective makes the calibration insensitive to the joint displacement of the trap and bead. Here, in addition, we take advantage of the very well contrasted interference rings observed in this specific plane to extract with good accuracy the height of the trapped bead. We thus map the viscous drag dependence close to the surface and reach an agreement between four calibration techniques, on an accurate value of the trap stiffness down to 2 micron distance to the surface and up to 600 mW of laser power. Combining this detection scheme with phase contrast microscopy, we show that the phase ring in the back focal plane of the objective overlaps with the interference rings and must be deported in a conjugate plane on the imaging path.
在背向散射光中使用干涉环精确校准靠近玻璃表面的光镊
检测反向反射的陷阱光束是测量光学镊子施加在生物系统上的力的一种方便方法,使顶部可以自由接触样品,并使其更容易与荧光和相位对比成像相结合。在显微镜物镜的后焦平面中这样做使得校准对捕获器和珠的联合位移不敏感。此外,在这里,我们利用在该特定平面中观察到的对比度非常好的干涉环,以良好的精度提取捕获珠的高度。因此,我们绘制了接近表面的粘性阻力依赖关系图,并在四种校准技术之间达成了一致,即阱刚度的精确值低至表面2微米的距离和高达600mW的激光功率。将这种检测方案与相位对比显微镜相结合,我们表明物镜后焦平面中的相位环与干涉环重叠,并且必须在成像路径上的共轭平面中被驱逐。
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来源期刊
CiteScore
2.40
自引率
0.00%
发文量
12
审稿时长
5 weeks
期刊介绍: Rapid progress in optics and photonics has broadened its application enormously into many branches, including information and communication technology, security, sensing, bio- and medical sciences, healthcare and chemistry. Recent achievements in other sciences have allowed continual discovery of new natural mysteries and formulation of challenging goals for optics that require further development of modern concepts and running fundamental research. The Journal of the European Optical Society – Rapid Publications (JEOS:RP) aims to tackle all of the aforementioned points in the form of prompt, scientific, high-quality communications that report on the latest findings. It presents emerging technologies and outlining strategic goals in optics and photonics. The journal covers both fundamental and applied topics, including but not limited to: Classical and quantum optics Light/matter interaction Optical communication Micro- and nanooptics Nonlinear optical phenomena Optical materials Optical metrology Optical spectroscopy Colour research Nano and metamaterials Modern photonics technology Optical engineering, design and instrumentation Optical applications in bio-physics and medicine Interdisciplinary fields using photonics, such as in energy, climate change and cultural heritage The journal aims to provide readers with recent and important achievements in optics/photonics and, as its name suggests, it strives for the shortest possible publication time.
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