Potential probiotic properties and molecular identification of lactic acid Bacteria isolated from fermented millet porridge or ragi koozh and jalebi batter

Q4 Biochemistry, Genetics and Molecular Biology

Animal Gene Pub Date : 2022-09-01 DOI:10.1016/j.angen.2022.200134

Manoharan Manovina , Balasubramanian Thamarai Selvi , Ragothaman Prathiviraj , Joseph Selvin

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引用次数: 10

Abstract

The consumer market has significantly increased in the search for nutritional and probiotic formulated products. In the present study, probiotic characterization parameters have been evaluated for the bacteria isolated from fermented millet porridge or ragi koozh and jalebi batter. Initially, 30 colonies were randomly picked and screened for lactic acid production. Lactic acid-producing 13 isolates showing gram-positive cell wall, non-spore-forming capacity and catalase-negative strains were subjected to probiotic characterization. Among 13 isolates, 2 isolates S9 and S13 exhibited good tolerance towards in-vitro characterization parameters such as acid and bile tolerance, adhesion to hydrocarbons, autoaggregation, co-aggregation, antibacterial activity, and safety measures. The potential probiotic strains S9 and S13 were identified by molecular methods, and evolutionary imprints and submitted to GenBank as Weisella cibaria and Enterococcus lactis. Results showed the efficacy of probiotic characteristics of isolates S9 and S13 which can be used for the formulation of probiotic products.

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发酵小米粥、腊肠和腊肠面糊中乳酸菌的潜在益生菌特性及分子鉴定

消费者市场对营养和益生菌配方产品的需求显著增加。本研究对从发酵小米粥或腊肠、加勒比面糊中分离得到的益生菌进行了鉴定。最初，随机挑选30个菌落进行乳酸生成筛选。对产乳酸的13株细胞壁革兰氏阳性、无孢子形成能力和过氧化氢酶阴性的菌株进行益生菌鉴定。在13株分离菌株中，2株S9和S13对酸和胆汁耐受性、对烃类的粘附、自聚集、共聚集、抗菌活性和安全措施等体外表征参数表现出良好的耐受性。通过分子方法鉴定了潜在的益生菌菌株S9和S13，并建立了进化印迹，分别作为锡巴魏氏菌和乳酸肠球菌提交给GenBank。结果表明，分离菌株S9和S13具有良好的益生菌特性，可用于益生菌制剂的研制。

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Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.