Quercetin 3 Rutinoside Facilitates Protection Against Radiation Induced Genotoxic and Oxidative Damage A Study in C57bl 6 Mice

Q3 Pharmacology, Toxicology and Pharmaceutics
S. Verma, A. Dutta
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引用次数: 5

Abstract

Radiation-induced oxidative stress and haematopoietic genomic instability is the major concern during planned or unplanned exposure. Use of the natural phytochemicals is an emerging strategy to prevent from the harmful effects of radiation. In the current investigation, Quercetin 3-Rutinoside (Q-3-R), a polyphenolic bioflavonoid, has been evaluated against gamma radiation (2Gy) induced genotoxic damage and oxidative imbalance in mice. Mice were administered with Q-3-R (10mg/kg body weight) 1hr prior to irradiation and evaluated for its antioxidant potential. Anti-genotoxic potential was assessed in terms of chromosomal aberrations in bone marrow cells. Findings revealed that Q-3-R had very high reducing potential, effectively scavenged 1,1-Diphenyl-2-picryl hydrazyl (DPPH) and hydrogen peroxide radicals, chelated metal ions and inhibited lipid peroxidation in a dose dependant manner. The glutathione (GSH) levels were found elevated (p<0.05), while reduced malondialdehyde (MDA) levels were seen in blood and liver tissues of Q-3-R pretreated mice. Significant (p<0.01) reduction in Reactive Oxygen Species (ROS) levels and radiation induced aberrations (dicentrics, rings, fragments, end to end association, robertsonian translocation) following Q-3-R pretreatment was found in bone marrow cells. The present findings demonstrate that Q-3-R can effectively minimise radiation-induced genotoxic and oxidative damages and can be explored further to be used as a potent radioprotector in humans.
槲皮素- 3芦丁苷对C57bl - 6小鼠辐射诱导的基因毒性和氧化损伤的保护作用研究
辐射诱导的氧化应激和造血基因组不稳定是计划内或计划外暴露期间的主要问题。使用天然植物化学物质是防止辐射有害影响的一种新兴策略。在目前的研究中,槲皮素3-芸香苷(Q-3-R)是一种多酚类生物类黄酮,已被评估可对抗γ辐射(2Gy)诱导的小鼠遗传毒性损伤和氧化失衡。小鼠在辐照前1小时服用Q-3-R(10mg/kg体重),并评估其抗氧化潜力。通过骨髓细胞染色体畸变来评估抗基因毒性的潜力。研究结果表明,Q-3-R具有很高的还原潜力,能有效清除1,1-二苯基-2-苦基肼(DPPH)和过氧化氢自由基,螯合金属离子,并以剂量依赖的方式抑制脂质过氧化。Q-3-R预处理小鼠的血液和肝组织中谷胱甘肽(GSH)水平升高(p<0.05),而丙二醛(MDA)水平降低。在骨髓细胞中发现Q-3-R预处理后活性氧(ROS)水平和辐射诱导的畸变(双心室、环、片段、端到端结合、罗伯逊易位)显著降低(p<0.01)。目前的研究结果表明,Q-3-R可以有效地将辐射诱导的遗传毒性和氧化损伤降至最低,并可以进一步探索作为一种有效的人体辐射保护剂。
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来源期刊
Defence Life Science Journal
Defence Life Science Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
0.80
自引率
0.00%
发文量
26
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