Promotion effects of salt-alkali on ammonia volatilization in a coastal soil

Abstract

Coastal ecosystems are highly susceptible to salt-related problems due to their formation process and geographical location. As such ecosystems are the most accessible land resources on Earth, clarifying and quantifying the effects of salt-alkali conditions on N concentration and ammonia (NH₃) volatilization are pivotal for promoting coastal agricultural productivity. The challenge in establishing this effect is to determine how salt-alkali conditions impact NH₃ volatilization through direct or indirect interactions. An incubation experiment using a coastal soil from a paddy farmland, combined with the structural equation modeling (SEM) method, was conducted to reveal the net effects of salt-alkali on NH₃ volatilization and the role of environmental and microbial factors in mutual interaction networks. The specific experimental design consisted of four salt treatments (S1, S2, S3, and S4: 1‰, 3‰, 8‰, and 15‰ NaCl by mass of soil, respectively), four alkaline treatments (A1, A2, A3, and A4: 0.5‰, 1‰, 3‰, and 8‰ NaHCO₃ by mass of soil, respectively) and a control without NaCl or NaHCO₃ addition (CK), and each treatment had three urea concentrations (N1, N2, and N3: 0.05, 0.10, and 0.15 g N kg^-1 soil, respectively) and three replicates. At the N1, N2, and N3 levels, NH₃ volatilization increased by 9.31%–34.98%, 3.07%–26.92%, and 2.99%–43.61% as the NaCl concentration increased from 1‰ to 15‰, respectively, compared with CK. With an increase in the NaHCO₃ concentration from 0.5‰ to 8‰, NH₃ volatilization increased by 8.36%–56.46%, 5.49%–30.10%, and 30.72%–73.18% at the N1, N2, and N3 levels, respectively, compared with CK. According to the SEM method, salinity and alkalinity had positive direct effects on NH₃ volatilization, with standardized path coefficients of 0.40 and 0.19, respectively. Considering the total effects (net positive and negative effects) in the SEM results, alkalinity had a greater influence than salinity (total standardized coefficient 0.104 > 0.086). Nitrogen concentrations in the incubation system showed a direct positive effect on NH₃ volatilization (standardized path coefficient = 0.78), with an obvious decrease under elevated salinity and alkalinity levels. Additionally, gene abundances of nitrogen-transforming microbes indirectly increased NH₃ volatilization (total indirect standardized coefficient = 0.31). Our results indicated that potential NH₃ emissions from coastal saline areas could be enhanced more by soil alkalization than by salinization.