Comparing Fluidized Bed Spray-Coating and Spray-Drying Encapsulation of Non-Spore-Forming Gram-Negative Bacteria

IF 1.3 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ryan Kawakita, J. Leveau, T. Jeoh
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引用次数: 4

Abstract

10 Microencapsulation of plant-beneficial bacteria for use as biopesticides is a growing area of 11 interest in the search for alternatives to harmful chemical pesticides. In this study, we assessed 12 the microencapsulation of the Gram-negative, non-spore-forming plant-beneficial bacterial strain 13 Collimonas arenae Cal35 in novel cross-linked alginate microcapsules (CLAMs) formed by 14 spray-drying, and in novel cross-linked alginate matrix shell (CLAMshell) particles formed by 15 fluidized bed spray-coating. Survival of Cal35 was 10-fold greater in CLAMs than in 16 CLAMshells. During individual stress tests, Cal35 was found to be more tolerant to high 17 temperatures than to desiccation. This is consistent with the greater survival of Cal35 when 18 encapsulated by spray-drying, which is a process that uses high inlet temperature and short 19 exposure times to dry conditions. The particle diameter of CLAMs ranged from 5 to 20 µm, 20 indicating potential for spray applications. CLAMshell particle diameters were between 250 and 21 300 µm, suggesting potential for seed coatings.
非孢子形成革兰氏阴性菌的流化床喷涂与喷雾干燥包封的比较
在寻找有害化学农药的替代品时,将有益植物的细菌微胶囊化用作生物农药是一个日益受到关注的领域。在这项研究中,我们评估了革兰氏阴性,不形成孢子的植物有益细菌菌株13 Collimonas arenae Cal35在14喷雾干燥形成的新型交联藻酸盐微胶囊(CLAMs)和15流化床喷雾涂层形成的新型交联藻酸盐基质壳(CLAMshell)颗粒中的微胶囊化情况。Cal35在蛤壳中的存活率是16蛤壳的10倍。在个体压力测试中,发现Cal35对高温的耐受性比干燥更强。这与使用喷雾干燥封装Cal35时的更高存活率是一致的,喷雾干燥是一种使用高入口温度和短暴露时间在干燥条件下的过程。CLAMs的颗粒直径范围从5到20µm, 20表明喷雾应用的潜力。蛤壳颗粒直径在250 ~ 21,300µm之间,表明种子包衣的潜力。
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来源期刊
Industrial Biotechnology
Industrial Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
2.20
自引率
0.00%
发文量
44
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