{"title":"Exploring natural hybridizations among Asplenium ruprechtii and related taxa in Korea","authors":"Chang Shook Lee, S. Yeau, Kyong-Sook Chung","doi":"10.11110/KJPT.2019.49.2.127","DOIUrl":null,"url":null,"abstract":"The purported four hybrid origins of Asplenium in Korea were tested based on morphological, cytological and DNA sequence data. Asplenium castaneo-viride, A. × uiryeongse, A. × montanus, and A. × kitazawae share several morphological characteristics with the Asian walking fern A. ruprechtii and related taxa as parents and show a sympatric distribution with the putative parents, raising the possibility of hybrid origins: A. castaneo-viride (A. ruprechtii and A. incisum), A. × uiryeongse (A. ruprechtii and A. pekinense), A. × montanus (A. ruprechtii, A. trichomanes, and A. incisum), and A. × kitazawae (A. ruprechtii and A. sarelii). We investigated flow cytometry and chloroplast DNA sequence data (rbcL, rps4-trnS, and rps4-trnS intergenic spacer) to clarify the hybridization and origin of each hybrid. In the flow cytometry analyses, A. ruprechtii shows diploid (2x) only, whereas A. castaneo-viride (3x, 4x), A. × uiryeongse (3x), A. × montanus (3x, 4x), and A. × kitazawae (2x, 4x) exhibit polyploidy, suggesting hybrid events along speciation. The rbcL and rps4-trnS and rps4-trnS intergenic spacer data suggest that A. ruprechtii is one the maternal ancestors of all four hybrids. In addition, the rps4-trnS and rps4-trnS intergenic spacer data indicate that A. incisum is also the maternal ancestor of A. × kitazawae and A. × montanus, proposing multiple hybridization events for these two hybrids. In A. × montanus, morphological features such as the leaf forms and sympatric distributions of the species also support the multimaternal hypothesis, but the morphological features of A. × kitazawae must be examined with consideration of hybrid events. To clarify the complex hybrid evolutionary lineages of the four Asplenium hybrids, further research with taxon sampling and molecular markers should be conducted.","PeriodicalId":52232,"journal":{"name":"KOREAN JOURNAL OF PLANT TAXONOMY","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"KOREAN JOURNAL OF PLANT TAXONOMY","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11110/KJPT.2019.49.2.127","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 1
Abstract
The purported four hybrid origins of Asplenium in Korea were tested based on morphological, cytological and DNA sequence data. Asplenium castaneo-viride, A. × uiryeongse, A. × montanus, and A. × kitazawae share several morphological characteristics with the Asian walking fern A. ruprechtii and related taxa as parents and show a sympatric distribution with the putative parents, raising the possibility of hybrid origins: A. castaneo-viride (A. ruprechtii and A. incisum), A. × uiryeongse (A. ruprechtii and A. pekinense), A. × montanus (A. ruprechtii, A. trichomanes, and A. incisum), and A. × kitazawae (A. ruprechtii and A. sarelii). We investigated flow cytometry and chloroplast DNA sequence data (rbcL, rps4-trnS, and rps4-trnS intergenic spacer) to clarify the hybridization and origin of each hybrid. In the flow cytometry analyses, A. ruprechtii shows diploid (2x) only, whereas A. castaneo-viride (3x, 4x), A. × uiryeongse (3x), A. × montanus (3x, 4x), and A. × kitazawae (2x, 4x) exhibit polyploidy, suggesting hybrid events along speciation. The rbcL and rps4-trnS and rps4-trnS intergenic spacer data suggest that A. ruprechtii is one the maternal ancestors of all four hybrids. In addition, the rps4-trnS and rps4-trnS intergenic spacer data indicate that A. incisum is also the maternal ancestor of A. × kitazawae and A. × montanus, proposing multiple hybridization events for these two hybrids. In A. × montanus, morphological features such as the leaf forms and sympatric distributions of the species also support the multimaternal hypothesis, but the morphological features of A. × kitazawae must be examined with consideration of hybrid events. To clarify the complex hybrid evolutionary lineages of the four Asplenium hybrids, further research with taxon sampling and molecular markers should be conducted.
在形态学、细胞学和DNA序列数据的基础上,对韩国的四个杂种起源进行了测试。astplenium castanoe -viride、a . x uiryeongse、a . x montanus和a . x kitazawae与亚洲行走蕨类a . ruprechtii及其相关类群具有相同的形态特征,并与推定的亲本具有同域分布,提出了杂交起源的可能性;绿斑姬斑姬螨(A. ruprechtii and A. incisum)、乌林英斑姬螨(A. ruprechtii and A. pekinense)、山斑姬螨(A. ruprechtii、A. trichomanes和A. incisum)、北泽斑姬螨(A. ruprechtii and A. sarelii)。我们利用流式细胞术和叶绿体DNA序列数据(rbcL、rps4-trnS和rps4-trnS基因间间隔)来澄清每个杂种的杂交和起源。在流式细胞术分析中,A. ruprechtii仅表现为二倍体(2x),而A. castanee -viride (3x, 4x)、A. x uiryeongse (3x)、A. x montanus (3x, 4x)和A. x kitazawae (2x, 4x)表现为多倍体,表明在物种形成过程中存在杂交事件。rbcL、rps4-trnS和rps4-trnS基因间间隔数据表明,鲁氏单胞螨是这4个杂种的母系祖先之一。此外,rps4-trnS和rps4-trnS基因间间隔数据表明,A. incisum也是A. x kitazawae和A. x montanus的母系祖先,说明这两个杂交品种存在多重杂交事件。在A. x montanus中,叶片形态和同域分布等形态特征也支持多母假说,但A. x kitazawae的形态特征必须考虑杂交事件。为明确4个阿斯勒属杂交种复杂的杂交进化谱系,还需进一步进行分类群取样和分子标记研究。