Effect of Different Ultrasound-Assisted Process Modes on Extraction Yield and Molecular Characteristics of Pepsin-Soluble Collagen from Asian Bullfrog Skin

IF 5.3 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food and Bioprocess Technology Pub Date : 2023-05-17 DOI:10.1007/s11947-023-03118-w

Sylvia Indriani, Soottawat Benjakul, Tran Hong Quan, Azis Boing Sitanggang, Manat Chaijan, Pensiri Kaewthong, Tanyamon Petcharat, Supatra Karnjanapratum

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引用次数: 1

Abstract

Different modes of ultrasound-assisted process (UAP) enhance pepsin-soluble collagen (PSC) extraction and affect characteristics of PSC. This study aimed to evaluate the efficacy and characteristics of PSC extracted from Asian bullfrog skin using different modes of UAP (pretreatment (UP) and simultaneous treatment (US)) and ultrasonication times (15, 30, and 45 min). Both UP and US increased solid yield and recovery compared with control (without UAP). The α-amino group content and surface hydrophobicity of PSC varied according to the mode used. The protein pattern revealed the type I collagen for all PSC samples. Fourier-transform infrared spectroscopy and circular dichroism spectra indicated that UAP has a significant effect on the molecular structure, with a negligible impact on the integrity of the triple helix structure. Nevertheless, the UAP conditions applied in this study affected the structural stringency by lowering the thermal stability. UP at 30 min and US at 45 min, which displayed high extraction recovery and satisfactory triple helix structure, were selected for further analyses. The difference in isoelectric point and amino acid composition was also observed between each sample. Conclusively, both UP and US applied for appropriate time periods successfully enhanced the PSC extraction efficiency with various molecular characteristics of collagen.

Graphical Abstract

Abstract Image

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不同超声辅助处理方式对亚洲牛蛙皮肽溶性胶原提取率及分子特性的影响

不同的超声辅助工艺(UAP)对胃蛋白酶可溶性胶原蛋白(PSC)的提取有促进作用，并影响PSC的特性。本研究旨在评价不同UAP模式(预处理(UP)和同时处理(US))和超声时间(15、30和45 min)下提取亚洲牛蛙皮PSC的功效和特性。与对照(不含UAP)相比，UP和US均提高了固体产量和回收率。PSC的α-氨基含量和表面疏水性随模式的不同而不同。蛋白模式显示所有PSC样品均为I型胶原蛋白。傅里叶变换红外光谱和圆二色性光谱表明，UAP对分子结构有显著影响，对三螺旋结构完整性的影响可以忽略不计。然而，本研究中应用的UAP条件通过降低热稳定性来影响结构的刚性。选择30 min的UP和45 min的US，提取回收率高，三螺旋结构满意，进一步分析。各样品的等电点和氨基酸组成也存在差异。综上所述，在适当的时间内使用UP和US均可提高具有不同胶原分子特征的PSC的提取效率。图形抽象

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来源期刊

Food and Bioprocess Technology 农林科学-食品科技

CiteScore

9.50

自引率

19.60%

发文量

200

审稿时长

2.8 months

期刊介绍： Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community. The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.