Effect of Ascorbic Acid on Collagen and Some Inflammatory Mediators in Rats

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Ankara Universitesi Veteriner Fakultesi Dergisi Pub Date : 2022-04-18 DOI:10.33988/auvfd.1019201

Sibel H. Isin, N. Yazıhan, Burcu Kesikli, D. Biriken, S. Akdas, Ferda Topal Çelikkan, B. Bakırarar, Pelin Aribal-Ayral

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引用次数: 0

Abstract

Musculoskeletal injuries as a kind of traumas that the human body is exposed to, adversely affect the quality of life and workforce of individuals due to restriction of movement function. This study was aimed to evaluate the effects of dose-dependent ascorbic acid administration on the repair process after gastrocnemius muscle injury in rats. In this study, 5-month-old 66 male Wistar Albino rats were used and rats were randomized to 6 groups (n = 11/group). A linear incision was made in the gastrocnemius muscle of thirty-three animals included in the muscle injury groups. Ascorbic acid (5-10 mg/kg/day) was administered to the four groups intraperitoneally just after surgery once a day. Animals were sacrificed twenty-one days later. Blood and tissue samples were used for cytokine, collagen, and histological measurements. It was found that ascorbic acid reduced serum IL-6 and muscle tissue TNF-α levels, and increased liver tissue IL-10 levels. Muscle tissue collagen levels were not statistically different between the groups in parallel with our histological results. In our study, it was demonstrated that vitamin C has effects on inflammatory mediators during muscle tissue repair to explain mechanism detailed molecular analyzes are needed.

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抗坏血酸对大鼠胶原蛋白及部分炎症介质的影响

肌肉骨骼损伤作为人体暴露的一种创伤，由于运动功能的限制，严重影响了个体的生活质量和劳动能力。本研究旨在评价剂量依赖性抗坏血酸给药对大鼠腓肠肌损伤后修复过程的影响。实验选用5月龄Wistar Albino雄性大鼠66只，随机分为6组，每组11只。将33只动物作为肌肉损伤组，在腓肠肌上做直线切口。4组患者术后即刻腹腔注射抗坏血酸5 ~ 10 mg/kg/d，每日1次。21天后，动物被宰杀。血液和组织样本用于细胞因子，胶原蛋白和组织学测量。结果发现，抗坏血酸降低血清IL-6和肌肉组织TNF-α水平，升高肝脏组织IL-10水平。肌肉组织胶原蛋白水平在组间无统计学差异，与我们的组织学结果平行。在我们的研究中，维生素C对肌肉组织修复过程中的炎症介质有影响，需要详细的分子分析来解释其机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ankara Universitesi Veteriner Fakultesi Dergisi 农林科学-兽医学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ankara Üniversitesi Veteriner Fakültesi Dergisi is one of the journals’ of Ankara University, which is the first well-established university in the Republic of Turkey. Research articles, short communications, case reports, letter to editor and invited review articles are published on all aspects of veterinary medicine and animal science. The journal is published on a quarterly since 1954 and indexing in Science Citation Index-Expanded (SCI-Exp) since April 2007.