Investigations of Cardiac Functions and Organ Damages in Neonatal Calves with Suscepted Sepsis

Abstract

ABSTRACT The aims of the present study were to determine cardiac dysfunction and organ damages in neonatal calves with suspected sepsis. In the study, 20 neonatal calves with suscepted sepsis and 10 clinically healthy neonatal calves were used. Sera were collected from each animal and used to determine cardiac troponin I (cTnI), N-terminal pro-brain natriuretic peptide (NT-proBNP), creatine kinase-myocardial band (CK-MB), lactate dehydrogenase (LDH) and histone H3 levels. In the study, serum levels of cTnI (p<0.05), NT-proBNP (p<0.05), CK-MB (p<0.001), LDH (p<0.001) and histone H3 (p<0.01) were significantly higher in calves with suspected sepsis than those of control calves. Results of the present study indicate that, cardiac functions are impaired, and cardiac and other organ damages are occurred in sepsis suspected neonatal calves. This is the first report that cardiac dysfunction and sepsis-related organ damages were indicated by the elevated NT-proBNP and histone H3 in neonatal calves with suspected sepsis. It is suggestive that, cTnI and NT-proBNP to detect cardiac dysfunction and histone H3 to detect organ damages can be useful as diagnostic and prognostic biomarkers in sepsis suspected calves. Key words: Calves, cardiac biomarkers, Sepsis. OZ ABSTRACT Bu calismada sepsis supheli neonatal buzagilarda kalp fonksiyon bozukluklari ve organ hasarinin belirlenmesi amaclanmistir. Calismada sepsis supheli 20 ve klinik olarak saglikli 10 adet neonatal buzagi kullanildi. Her hayvandan serum ornekleri toplandi ve bu ornekler kardiak troponin I (CTnI), N-terminal pro-natriuretik peptid (NT-proBNP), kreatinin kinaz-myokardial band (CK-MB), laktat dehidrojenaz (LDH) ve histon H3 seviyelerinin belirlenmesinde kullanildi. Calismada, sepsis supheli buzagilarin serum cTnI (p<0.05), NT-proBNP (p<0.05), CK-MB (p<0.001), LDH (p<0.001) ve histon H3 (p<0.01) duzeylerinin kontrol grubununkilere gore istatistiksel olarak anlamli duzeyde yuksek oldugu belirlendi. Calismada elde edilen sonuclar, sepsis supheli buzagilarda kalp fonksiyonlarinin bozuldugunu, kalp ve diger organlarda hasarin olustugunu ortaya koymustur. Bu calisma ile ilk defa sepsis supheli buzagilarda kalp fonksiyon bozuklugu ve sepsis iliskili organ hasarlari artan serum NT-proBNP ve histon H3 seviyeleri ile belirlenmistir. Calismada elde edilen sonuclara gore, sepsis supheli buzagilarda cTnI ve NT-proBNP kalp fonksiyon bozuklugunun belirlenmesinde ve histon H3 ise organ hasarlarinin belirlenmesinde yararli prognostik ve diyagnostik biyomarkirlar olarak onerilebilir. Anahtar Kelimeler: buzagi, kardiak biyomarkirlar, sepsis