Heba Ahmed Mohamed, Gamal Fadl Mahmoud Gad, Mona Fattouh Mohamed, Hend Harby Ahmed, Ameer Effat Elfarash, Nahed Fathallah Fahmy
{"title":"Genotyping of carbapenem resistant Acinetobacter baumannii isolated from Egyptian patients","authors":"Heba Ahmed Mohamed, Gamal Fadl Mahmoud Gad, Mona Fattouh Mohamed, Hend Harby Ahmed, Ameer Effat Elfarash, Nahed Fathallah Fahmy","doi":"10.21608/nrmj.2022.276319","DOIUrl":null,"url":null,"abstract":"Acinetobacter baumannii has recently been known as a major cause of hospital- and community-acquired infections. Carbapenem resistant A. baumanni (CRAB) has been recorded to be resistant to nearly all antibiotics, including the last resort antibiotics; carbapenems. This study aimed to detect the carbapenem resistance levels and mechanisms, in addition to the genotyping of A. baumanni in Upper Egypt. About 200 clinical samples were collected from different wards of Sohag University Hospital, Egypt, from which 20 A. baumannii isolates were recovered and then identified using conventional methods and Polymerase Chain Reaction (PCR). Antibiotic sensitivity testing was carried out using the Disk diffusion method, followed by PCR testing of the common carbapenemase-encoding genes, including OXA-51 , OXA-58 , KPC , GES , IMP , NDM","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Novel Research in Microbiology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/nrmj.2022.276319","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1
Abstract
Acinetobacter baumannii has recently been known as a major cause of hospital- and community-acquired infections. Carbapenem resistant A. baumanni (CRAB) has been recorded to be resistant to nearly all antibiotics, including the last resort antibiotics; carbapenems. This study aimed to detect the carbapenem resistance levels and mechanisms, in addition to the genotyping of A. baumanni in Upper Egypt. About 200 clinical samples were collected from different wards of Sohag University Hospital, Egypt, from which 20 A. baumannii isolates were recovered and then identified using conventional methods and Polymerase Chain Reaction (PCR). Antibiotic sensitivity testing was carried out using the Disk diffusion method, followed by PCR testing of the common carbapenemase-encoding genes, including OXA-51 , OXA-58 , KPC , GES , IMP , NDM
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