Characteristic Test and Antihyperuricemia Activity of Ethanol Extract Stem of Etlingera rubroloba A.D Poulsen In vitro

MedULA Pub Date : 2022-05-25 DOI:10.46496/medula.v9i2.25290

A. Jabbar, I. Sahidin, Khansa Rafida Triaqilah, M. Mubarak, M. Yusuf

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Abstract

Background: Etlingera rubroloba (E. rubroloba) A.D Poulsen is one of the endemic plants of Southeast Sulawesi, which has the largest species in the world. Local people widely use this plant as traditional medicine. Many of these plant species have been previously reported as antioxidants, antibacterials, and others. However, for E. rubroloba, there are still very few reports from a pharmacological aspect, especially anthiperuricemia. Objective: This study was conducted to determine the characteristics and activity of the ethanol extract of the stem of E. rubroloba A.D Poulsen in vitro. Methods: The stems of E. rubroloba A.D Poulsen were extracted with ethanol solvent by maceration, then the filtrate obtained was evaporated using an evaporator to get an ethanol extract. Furthermore, the extract characterization test was carried out, including water content, ash content, water-soluble extract content, and ethanol-soluble extract content. Then the antihyperuricemia activity test in vitro using the Xanthine Oxidase (XO) kit. Results: The results of the characteristic test showed that the ethanolic extract of E. rubroloba contained 5.15% water content, 5.485% ash content, 55.2% water-soluble extract content, and 56.52% ethanol-soluble extract content. Furthermore, the antihyperuricemic activity of the ethanol extract of E. rubroloba was IC50 48.59 ± 0.03 g/mL and Allopurinol 42.21 ± 0.02 g/mL as positive controls. Conclusion: E. rubroloba stem ethanol extract has characterization results according to the literature, has antihyperuricemia activity, and is a reference for the development of traditional drugs

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杨梅乙醇提取物茎的体外特性及抗高尿酸血症活性

背景：红叶Etlingera rubloba（E.rubloba）A.D Poulsen是苏拉威西岛东南部的特有植物之一，该岛拥有世界上最大的物种。当地人广泛使用这种植物作为传统药物。这些植物物种中的许多以前被报道为抗氧化剂、抗菌剂和其他。然而，对于红热病，从药理学方面的报道仍然很少，尤其是黄热病。目的：本研究旨在测定红宝茎乙醇提取物的体外特性和活性。方法：用乙醇溶剂浸渍法提取红花茎，然后用蒸发器蒸发滤液，得到乙醇提取物。此外，还进行了提取物的表征测试，包括水含量、灰分、水溶性提取物含量和乙醇溶性提取物的含量。然后用黄嘌呤氧化酶（XO）试剂盒进行体外抗高血压活性试验。结果：特性试验结果表明，红叶赤藓乙醇提取物含水量5.15%，灰分5.485%，水溶性提取物55.2%，乙醇溶性提取物56.52%。此外，作为阳性对照，红曲霉乙醇提取物的抗高血压活性为IC50 48.59±0.03 g/mL和别嘌醇42.21±0.02 g/mL。结论：红曲霉茎乙醇提取物具有文献表征结果，具有抗高血压活性，可为传统药物的开发提供参考

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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MedULA MEDICINE, GENERAL & INTERNAL-

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