Henrique K. Noguchi , Sarbjeet Kaur , Luiza M. Krettli , Pankaj Singla , Jake McClements , Helena Snyder , Robert D. Crapnell , Craig E. Banks , Katarina Novakovic , Inderpreet Kaur , Jonas Gruber , James A. Dawson , Marloes Peeters
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引用次数: 2
Abstract
A portable test to rapidly determine levels of levodopa, the drug used to treat Parkinson's disease, can improve clinical management of the disease. In this study, screen-printed electrodes (SPEs) were modified with polymers to facilitate the electrochemical detection of levodopa. Cyclic voltammetry was used to deposit a thin layer of polyaniline on the electrode surface. Scanning electron microscopy revealed high surface coverage, which did not impact the electrode's conductivity. Differential pulse voltammetry measurements with the polyaniline-modified electrodes enabled the measurement of levodopa at physiologically relevant concentrations with discrimination between a common interferent (ascorbic acid) and a structurally similar compound (l-tyrosine). However, the use of the polymer layer did not permit differentiation between levodopa and dopamine; the only difference in these molecules is that levodopa has an amino acid moiety whereas dopamine has a free amine group. Density functional theory calculations demonstrated that aniline formed a hydrogen bond between the amino group of the monomer and the meta-hydroxyl group, which is present in both levodopa and dopamine, with similar binding energies (−53.36 vs −50.08 kJ mol−1). Thus, the polymer-functionalised SPEs are a valuable tool to measure compounds important in Parkinson's disease, but further refinement is needed to achieve selective detection.
期刊介绍:
The scope of Physics in Medicine consists of the application of theoretical and practical physics to medicine, physiology and biology. Topics covered are: Physics of Imaging Ultrasonic imaging, Optical imaging, X-ray imaging, Fluorescence Physics of Electromagnetics Neural Engineering, Signal analysis in Medicine, Electromagnetics and the nerve system, Quantum Electronics Physics of Therapy Ultrasonic therapy, Vibrational medicine, Laser Physics Physics of Materials and Mechanics Physics of impact and injuries, Physics of proteins, Metamaterials, Nanoscience and Nanotechnology, Biomedical Materials, Physics of vascular and cerebrovascular diseases, Micromechanics and Micro engineering, Microfluidics in medicine, Mechanics of the human body, Rotary molecular motors, Biological physics, Physics of bio fabrication and regenerative medicine Physics of Instrumentation Engineering of instruments, Physical effects of the application of instruments, Measurement Science and Technology, Physics of micro-labs and bioanalytical sensor devices, Optical instrumentation, Ultrasound instruments Physics of Hearing and Seeing Acoustics and hearing, Physics of hearing aids, Optics and vision, Physics of vision aids Physics of Space Medicine Space physiology, Space medicine related Physics.