Rapid electrochemical detection of levodopa using polyaniline-modified screen-printed electrodes for the improved management of Parkinson's disease

Q3 Medicine
Henrique K. Noguchi , Sarbjeet Kaur , Luiza M. Krettli , Pankaj Singla , Jake McClements , Helena Snyder , Robert D. Crapnell , Craig E. Banks , Katarina Novakovic , Inderpreet Kaur , Jonas Gruber , James A. Dawson , Marloes Peeters
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引用次数: 2

Abstract

A portable test to rapidly determine levels of levodopa, the drug used to treat Parkinson's disease, can improve clinical management of the disease. In this study, screen-printed electrodes (SPEs) were modified with polymers to facilitate the electrochemical detection of levodopa. Cyclic voltammetry was used to deposit a thin layer of polyaniline on the electrode surface. Scanning electron microscopy revealed high surface coverage, which did not impact the electrode's conductivity. Differential pulse voltammetry measurements with the polyaniline-modified electrodes enabled the measurement of levodopa at physiologically relevant concentrations with discrimination between a common interferent (ascorbic acid) and a structurally similar compound (l-tyrosine). However, the use of the polymer layer did not permit differentiation between levodopa and dopamine; the only difference in these molecules is that levodopa has an amino acid moiety whereas dopamine has a free amine group. Density functional theory calculations demonstrated that aniline formed a hydrogen bond between the amino group of the monomer and the meta-hydroxyl group, which is present in both levodopa and dopamine, with similar binding energies (−53.36 vs −50.08 kJ mol−1). Thus, the polymer-functionalised SPEs are a valuable tool to measure compounds important in Parkinson's disease, but further refinement is needed to achieve selective detection.

聚苯胺修饰丝网印刷电极快速电化学检测左旋多巴改善帕金森病的治疗
一种快速测定左旋多巴(用于治疗帕金森病的药物)水平的便携式检测方法可以改善该疾病的临床管理。在本研究中,用聚合物修饰丝网印刷电极(spe)以促进左旋多巴的电化学检测。用循环伏安法在电极表面沉积了一层薄薄的聚苯胺。扫描电子显微镜显示高表面覆盖率,这并不影响电极的导电性。用聚苯胺修饰电极的差分脉冲伏安法测量左旋多巴的生理相关浓度,并区分共同干扰物(抗坏血酸)和结构相似的化合物(l-酪氨酸)。然而,聚合物层的使用不能区分左旋多巴和多巴胺;这些分子的唯一区别是左旋多巴有一个氨基酸部分,而多巴有一个自由胺基。密度泛函理论计算表明,苯胺在单体氨基和间羟基之间形成氢键,其结合能相似(- 53.36 vs - 50.08 kJ mol−1),左旋多巴和多巴胺均存在这种氢键。因此,聚合物功能化spe是测量帕金森病中重要化合物的有价值的工具,但需要进一步改进以实现选择性检测。
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来源期刊
Physics in Medicine
Physics in Medicine Physics and Astronomy-Instrumentation
CiteScore
2.60
自引率
0.00%
发文量
9
审稿时长
12 weeks
期刊介绍: The scope of Physics in Medicine consists of the application of theoretical and practical physics to medicine, physiology and biology. Topics covered are: Physics of Imaging Ultrasonic imaging, Optical imaging, X-ray imaging, Fluorescence Physics of Electromagnetics Neural Engineering, Signal analysis in Medicine, Electromagnetics and the nerve system, Quantum Electronics Physics of Therapy Ultrasonic therapy, Vibrational medicine, Laser Physics Physics of Materials and Mechanics Physics of impact and injuries, Physics of proteins, Metamaterials, Nanoscience and Nanotechnology, Biomedical Materials, Physics of vascular and cerebrovascular diseases, Micromechanics and Micro engineering, Microfluidics in medicine, Mechanics of the human body, Rotary molecular motors, Biological physics, Physics of bio fabrication and regenerative medicine Physics of Instrumentation Engineering of instruments, Physical effects of the application of instruments, Measurement Science and Technology, Physics of micro-labs and bioanalytical sensor devices, Optical instrumentation, Ultrasound instruments Physics of Hearing and Seeing Acoustics and hearing, Physics of hearing aids, Optics and vision, Physics of vision aids Physics of Space Medicine Space physiology, Space medicine related Physics.
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