Molecular characteristics of the Porcine Epidemic Diarrhea Virus strains isolated in different regions of Ukraine

Q4 Biochemistry, Genetics and Molecular Biology

Biopolymers and Cell Pub Date : 2019-12-31 DOI:10.7124/bc.000a1b

D. Masiuk, V. Hlebeniuk, A. Kokariev, T. Vasylenko

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引用次数: 1

Abstract

Aim. Research on the molecular characteristics of the PEDV strains isolated in different regions of Ukraine. Methods. PEDV was detected and differentiated in biological material by PCR using the EZ-RED / TGE / PDCoV MPX 1.0 Realtime RT-PCR test system. The complete S gene was sequenced for comparative genotyping of the PEDV strains using PCR. Results. The diagnosis of PED was confirmed in the pig farms under investigation. The PED strains isolated in different regions of Ukraine have a high similarity (99 %) to the strains from North America in 2013-2014 and China in 2011-2012 and a lower similarity (90 %) with the strains circulating in Europe before 1995. The PEDV_Cherkasy_UA_17 strain was clustered into group 2 (cognate to the Chinese strain BJ-2011-1) in the North American clade II of the PEDV. The Ukrainian PEDV strain was phylogenetically clustered together with the highly virulent North American PEDV strains, but differed from the strains circulating in European countries. Conclusions. The PEDV strains isolated in various regions of Ukraine have a high (>99.5 %) degree of homology and are phylogenetically clustered with the highly virulent North American PEDV strains.

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乌克兰不同地区分离的猪流行性腹泻病毒株的分子特征

目标乌克兰不同地区分离的PEDV菌株的分子特征研究。方法。使用EZ-RED/TGE/PCoV MPX 1.0实时RT-PCR检测系统通过PCR在生物材料中检测并分化PEDV。使用PCR对完整的S基因进行测序以进行PEDV菌株的比较基因分型。后果PED的诊断在正在调查的养猪场得到了证实。在乌克兰不同地区分离到的PED菌株与2013-2014年北美和2011-2012年中国的菌株具有较高的相似性（99%），与1995年之前在欧洲流行的菌株具有较低的相似度（90%）。PEDV_Cherkasy_UA_17菌株在PEDV的北美分支II中聚类为第2组（与中国菌株BJ-2011-1同源）。乌克兰PEDV菌株与北美高毒力PEDV菌株在系统发育上聚在一起，但与欧洲国家流行的菌株不同。结论。在乌克兰不同地区分离的PEDV菌株具有高度（>99.5%）的同源性，并且在系统发育上与高毒力的北美PEDV菌株聚类。

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来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.