Isolation and Characterization of Enterococcus faecium from Fermented Korean Soybean Paste with Antibacterial Effects

IF 3.3 3区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Fermentation-Basel Pub Date : 2023-08-14 DOI:10.3390/fermentation9080760

Ki-seok Han, Soyoung Park, A. Sathiyaseelan, Myeon-Hyeon Wang

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Abstract

In the present study, a total of eight Enterococcus faecium (OQ940301, OQ940302, OQ940303, OQ940304, OQ940305, OQ940308, OQ940309, and OQ940310) were isolated from soybean paste, a traditional Korean fermented food, and evaluated for their probiotic properties. The results showed that all the E. faecium strains survived in simulated human gastrointestinal conditions (4.1–5.59 log10 CFU/mL). In addition, the range of auto-aggregation was 5–25%, the hydrophobicity was around 94%, and it exhibited significant co-aggregation ability with Salmonella enterica and Staphylococcus aureus. However, all the isolates were shown to be resistant to Gentamycin. The bacterial cell-free supernatant showed antibacterial activity against S. enterica, Escherichia coli, Bacillus cereus, Listeria monocytogenes, and S. aureus. Furthermore, E. faecium exhibited potent anti-oxidant activity by scavenging 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radicals. In addition, safety was confirmed by evaluating the hemolytic activity in blood agar; none of the bacterial isolates showed hemolysis. These results demonstrated that E. faecium (OQ940301 and OQ940309) isolated from soybean paste showed a higher probiotic potential.

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发酵大豆酱中粪肠球菌的分离及抗菌性能研究

本研究从韩国传统发酵食品大酱中分离得到8株粪肠球菌(OQ940301、OQ940302、OQ940303、OQ940304、OQ940305、OQ940308、OQ940309和OQ940310)，并对其益生菌特性进行了评价。结果表明，在模拟人胃肠道条件(4.1 ~ 5.59 log10 CFU/mL)下，所有粪肠杆菌菌株均存活。自聚集范围为5 ~ 25%，疏水性在94%左右，与肠炎沙门氏菌、金黄色葡萄球菌表现出显著的共聚集能力。然而，所有分离株均显示对庆大霉素耐药。细菌无细胞上清液对肠链球菌、大肠杆菌、蜡样芽孢杆菌、单核细胞增生李斯特菌和金黄色葡萄球菌均有抑菌活性。此外，粪肠杆菌还能清除2,2′-氮基-双(3-乙基苯并噻唑-6-磺酸)二铵盐(ABTS)自由基，显示出较强的抗氧化活性。此外，通过对血琼脂的溶血活性评价，证实了其安全性;所有细菌分离株均未出现溶血现象。结果表明，从大豆酱中分离得到的粪肠杆菌(OQ940301和OQ940309)具有较高的益生菌潜力。

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