José Ortiz-Esquivel, J. A. Rosado-Aguilar, R. Rodríguez-Vivas, M. Torres-Castro, E. Gutiérrez-Ruíz, Arturo Bates-Acosta, F. Puerto
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引用次数: 2
Abstract
Objective. To determine the circulation of West Nile virus (WNV) in horses from the eastern Yucatán, Mexico. Materials and Methods. For convenience, 184 horses from 23 production units in the municipalities of Tizimín and Panabá, Yucatán, were studied. A blood serum sample was obtained from each studied horse and evaluated with an immunoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) to detect immunoglobulin M (IgM) against WNV. Additionally, positive reactor and suspect serum samples were analyzed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA. Results. Eight studied horses were seropositive (4.3%, 8/184) to WNV and two were suspects (1.1%, 2/184). All serum samples were negative by RT-PCR. Conclusions. The detection of IgM specific against WNV in the studied horses shows recent infections with the virus and indicates its circulation in eastern Yucatán, Mexico.
期刊介绍:
The Journal MVZ Córdoba is an open access international scientific journal financed and edited by the University of Córdoba (Colombia). The journal publishes quarterly, continuously in PDF, XML, Epub, original articles, literature reviews, brief communications and clinical cases, peer-reviewed (double-blind) in Spanish and English, which are related to the agricultural and veterinary sciences. The journal is directed to natural and legal persons of veterinary medicine, animal husbandry, public health, epidemiology, aquaculture, biology, basic biomedical sciences and biotechnology and constitutes a space for academic and scientific discussion around the work of professionals in Veterinary Medicine and Zootechnics. Four-monthly publication.
"The Journal MVZ Córdoba supports the policies for registration of clinical trials of the World Health Organization (WHO) and the International Committee of Medical Journal Editors (ICMJE), since it recognizes the importance of these initiatives for international registration and dissemination. of information about clinical studies, in open access. As a result, since 2007, the journal MVZ Córdoba only publishes clinical research articles that have received an identification number in one of the Clinical Trial Registries validated by the criteria established by WHO and ICMJE, whose addresses are available in the ICMJE website. The identification number is recorded at the end of the summary. "