Cadmium exposure induces testicular oxidative damage and histopathological changes in the freshwater leech Limnatis nilotica (Savigny, 1822): the protective role of salicylic acid
Ichrak Khaled, I. Saidi, R. Ahmed, R. Amari, W. Aldahmash, O. Pacioglu, N. Hfaiedh, A. Harrath
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Abstract
The present study examined the histopathological changes and some parameters of oxidative stress in the testes of the freshwater leech Limnatis nilotica, following their exposure to 50 µg l−1 of cadmium (Cd). The protective effects of salicylic acid (SA) in mitigating Cd-induced oxidative and histopathological injury were also investigated. We found that Cd exposure caused several histopathological changes in leech testes, such as vacuolisation of spermatogenic cysts which were strongly destroyed and disorganised. Biochemical study showed oxidative stress damage marked by a substantial increase in malondialdehyde and hydrogen peroxidase levels as well as disturbance in the activity of the leech antioxidant system. Interestingly, the testes from the Cd and SA leech-exposed group exhibited increased enzymatic and nonenzymatic antioxidant mechanism defenses, including superoxide dismutase, catalase, glutathione peroxidase, glutathione, oxidized glutathione, thiols, and ascorbic acid, in comparison with the testes of leeches exposed to Cd alone. Thus, SA supplementation considerably attenuated the histopathological lesions induced by Cd exposure, thereby restoring the normal appearance of the testes. In summary, the findings of this study indicate that SA treatment ameliorated the testicular histopathological and oxidative stress-induced damages caused by exposure to Cd through enhancing the antioxidant defense system.
本研究检测了淡水水蛭(Limnatis nilotica)睾丸暴露于50 μ g l - 1镉(Cd)后的组织病理学变化和氧化应激的一些参数。研究了水杨酸(SA)对cd诱导的氧化损伤和组织病理学损伤的保护作用。我们发现Cd暴露引起水蛭睾丸的一些组织病理学变化,如生精囊肿的空泡化,这些囊肿被强烈破坏和混乱。生化研究表明,氧化应激损伤的特征是丙二醛和过氧化氢酶水平的显著增加,以及水蛭抗氧化系统活性的紊乱。有趣的是,与单独暴露于Cd的水蛭相比,Cd和SA水蛭暴露组的睾丸表现出更高的酶和非酶抗氧化机制防御,包括超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽、氧化谷胱甘肽、硫醇和抗坏血酸。因此,SA的补充大大减轻了Cd暴露引起的组织病理损伤,从而恢复了睾丸的正常外观。综上所述,本研究结果表明,SA处理通过增强抗氧化防御系统,改善了镉暴露引起的睾丸组织病理和氧化应激损伤。
期刊介绍:
The African Journal of Aquatic Science is an international journal devoted to the study of the aquatic sciences, covering all African inland and estuarine waters. The Journal publishes peer-reviewed original scientific papers and short articles in all the aquatic science fields including limnology, hydrobiology, ecology, conservation, biomonitoring, management, water quality, ecotoxicology, biological interactions, physical properties and human impacts on African aquatic systems.