NEW PROMISING AGENTS FOR PHOTODYNAMIC THERAPY

Abstract

The problem of oncological diseases has acquired a social and medical character. In this regard, the rapid development of new drugs and methods, as well as modification of existing methods in order to increase their effectiveness, is currently required. The paper aims to develop and study two promising agents for photodynamic therapy. We discussed a new binary complex based on the widely used photosensitizer chlorin E6 with europium, which can solve one of the main disadvantages of drugs for photodynamic therapy: it can be used to treat deeper tumors and act more effectively by transferring luminescence energy between europium and chlorin E6. Our other agent was copper-containing chlorophyllin. Chlorophyllin is a natural component, which is always promising in the development of new drugs. The optical and fluorescent parameters of the agents were determined using physicochemical methods; their ability to generate reactive oxygen species in the presence of two fluorescent probes was studied: 2ʹ, 7ʹ-dichlorodihydrofluorescein and dihydrofluorescein. Thus, we showed the effectiveness of chlorophyllin in comparison with chlorin E6 by 1.92 times at 50 μg / ml E6 and showed the effectiveness of the binary complex even at the minimum concentration of 10 μg / ml. Further research in cell cultures will develop the ways of the possible use of new substances as photosensitizers.