Evaluation of Aspirin Effect on Candida Glabrata Isolates with Resistance to Azole Compounds by Real-Time PCR

Abstract

Materials and methods: Five C. glabrata isolates were collected from patients with candidal vulvovaginitis referred to clinic. The antibiotic susceptibility test of isolates for fluconazole was performed according to the CLSI M38-A2 standard protocol. A 64μg/ml aspirin and 500ug/ml and likewise 64μg/ml fluconazole were used for the MIC detection test. The RNA of isolates was prepared according to the manufacturer’s instruction. Synthesis of cDNA was conducted with the 1621K kit (Fermentase).. The RT-PCR reaction was set up for ERG3, ERG6 and ERG11 genes and the housekeeping gene Beta-actin (β-actin) was amplified as internal control.