Molecular species delimitation of the Asian chestnut gall wasp biocontrol agent released in Italy

IF 1.3 3区农林科学 Q2 ENTOMOLOGY

Insect Systematics & Evolution Pub Date : 2019-05-17 DOI:10.1163/1876312X-00002188

M. Montagna, E. Gonella, V. Mereghetti, G. Magoga, E. Ferrari, Marianna Pontini, C. Ferracini, A. Alma

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引用次数: 10

Abstract

Molecular species recognition and identification, based on the mitochondrial cox1 and on the nuclear ITS2, were performed on individuals of Torymus sinensis collected in Italy, on its close relative T. beneficus and on native torymids. The automatic-gap-discovery (ABGD) analyses correctly separate almost all morphospecies. On the basis of cox1, individuals of late-spring T. beneficus clustered with T. sinensis, and those identified as early-spring T. beneficus were recognized as a separate entity. Whereas, T. beneficus ecotypes clustered with T. sinensis on the basis of ITS2. Coalescent tree-based methods confirmed these results. The cox1-based recognition of early-spring T. beneficus as a separate phylospecies led us to conclude that this taxon deserves to be treated as a valid species, whereas individuals identified as late-spring T. beneficus might be considered as part of T. sinensis. Morphological identification and BLAST analyses confirmed that no T. beneficus was imported into Italy to control Dryocosmus kuriphilus.

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意大利发布的板栗瘿蜂生物防治剂的分子种划分

基于线粒体cox1和细胞核ITS2，对采集自意大利的中华托利木(Torymus sinensis)、其近缘种T. beneficus和本土托利木(Torymus sinensis)进行了分子物种识别和鉴定。自动间隙发现(ABGD)分析正确地分离了几乎所有的形态种。在cox1的基础上，晚春益螟个体与中华益螟聚集在一起，早春益螟个体被单独识别。而在ITS2的基础上，益螟生态型与中华绒螟聚集在一起。基于聚结树的方法证实了这些结果。基于cox - 1对早春春益天牛作为一个独立种的识别使我们得出结论，该分类群应该被视为一个有效的种，而晚春益天牛个体可能被认为是中华天牛的一部分。形态学鉴定和BLAST分析证实，意大利并没有为防治kuriphilus干蛾而输入有益蜱。

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来源期刊

Insect Systematics & Evolution 生物-进化生物学

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Insect Systematics & Evolution (ISE) publishes original papers on all aspects of systematic entomology and the evolutionary history of both extant and extinct insects and related groups. Priority is given to taxonomic revisions and phylogenetic studies employing morphological and molecular data. ISE also welcomes reviews and syntheses that can appeal to a wide community of systematic entomologists. Single species descriptions, regional checklists, and phylogenetic studies based on few taxa or single molecular markers will generally not be accepted.