Adaptation to the hottest spot on earth: Dietary ecology of an enigmatic desert gecko based on DNA metabarcoding

IF 2 2区 生物学 Q3 EVOLUTIONARY BIOLOGY
Mahdi Rajabizadeh, Jérôme Morinière, Hossein Rajaei
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Abstract

The Lut Desert, southeastern Iran, regularly has the largest contiguous area of surface temperatures above 65°C compared to anywhere else on Earth. Despite these harsh conditions, some specialized species thrive in this extreme habitat. Misonne's spider gecko, Rhinogecko misonnei de Witte, 1973 (Reptilia, Squamata, Gekkonidae) is an endemic species that is native to the central part of the Lut Desert. This research aimed to explore the dietary gut content and ecology of Misonne's spider gecko populations inhabiting the Lut Desert. A DNA metabarcoding approach was applied to obtain data from the stomach content of six collected specimens. A total of 147,451 amplicon reads representing a total of 94 OTU (=Operational Taxonomic Units) for the CO1-5P barcode region were sequenced. Of 94 OTUs, 50 (53.2%) identically matched ≥97% with BOLD and/or NCBI database and were identified at the genus level. Blasting in both databases revealed the presence of three phyla including Chordata (55.23%), Arthropoda (44.72%), and Annelida (0.05). 99.54% of Chordata DNA belonged to Rhinogecko misonnei mainly coming from stomach tissue of the examined specimens. Within the arthropods, 87.64% of the OTUs matched Insecta and 12.08% Arachnida. Our findings indicate that Rhinogecko misonnei is a generalist gecko that feeds on a variety of food items. About 81% of Rhinogecko food items in the central Lut are migratory insects that come from adjacent areas of the Lut District, although the arachnid food items originate from the central Lut. This study emphasizes the importance of the insect fauna of adjacent areas of the Lut Desert for the stability of the Desert food web.

适应地球上最热的地方:基于DNA代谢编码的神秘沙漠壁虎的饮食生态学
与地球上任何其他地方相比,伊朗东南部的卢特沙漠经常拥有最大的地表温度超过65°C的连续区域。尽管条件恶劣,一些特殊的物种在这个极端的栖息地茁壮成长。Misonne的蜘蛛壁虎,Rhinogecko misonnei de Witte, 1973(爬行纲,鳞片目,壁虎科)是卢特沙漠中部的一种特有物种。本研究旨在探讨生活在卢特沙漠的米松氏蜘蛛壁虎种群的肠道成分和生态。采用DNA元条形码方法从收集的6个标本的胃内容物中获取数据。共测序了CO1-5P条形码区域147,451个扩增子,共94个OTU (=Operational Taxonomic Units)。94个otu中,50个(53.2%)与BOLD和/或NCBI数据库的匹配度≥97%,在属水平上得到鉴定。在两个数据库中均发现有脊索动物门(55.23%)、节肢动物门(44.72%)和环节动物门(0.05)。99.54%的脊索动物DNA属于米索尼鼻壁虎,主要来自胃组织。节肢动物中,87.64%的OTUs匹配昆虫科,12.08%的OTUs匹配蜘蛛科。我们的研究结果表明,米索内犀牛壁虎是一种多面手,以各种食物为食。卢特中部约81%的Rhinogecko食物是来自卢特区邻近地区的迁徙昆虫,尽管蜘蛛类食物来自卢特中部。本研究强调了卢特沙漠邻近地区昆虫动物群对沙漠食物网稳定的重要性。
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来源期刊
CiteScore
5.60
自引率
0.00%
发文量
13
审稿时长
>12 weeks
期刊介绍: The Journal of Zoological Systematics and Evolutionary Research (JZSER)is a peer-reviewed, international forum for publication of high-quality research on systematic zoology and evolutionary biology. The aim of the journal is to provoke a synthesis of results from morphology, physiology, animal geography, ecology, ethology, evolutionary genetics, population genetics, developmental biology and molecular biology. Besides empirical papers, theoretical contributions and review articles are welcome. Integrative and interdisciplinary contributions are particularly preferred. Purely taxonomic and predominantly cytogenetic manuscripts will not be accepted except in rare cases, and then only at the Editor-in-Chief''s discretion. The same is true for phylogenetic studies based solely on mitochondrial marker sequences without any additional methodological approach. To encourage scientific exchange and discussions, authors are invited to send critical comments on previously published articles. Only papers in English language are accepted.
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