Morphological and molecular characterization of Pythium species from Punjab, Pakistan

IF 1 Q3 PLANT SCIENCES

Archives of Phytopathology and Plant Protection Pub Date : 2023-02-25 DOI:10.1080/03235408.2023.2184232

M. Ali, S. Afzaal, F. Sharif, S. W. Ali, H. Nawaz, W. Khan

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引用次数: 1

Abstract

Abstract The genus Pythium belongs to the soilborne Oomycetes. This research was conducted to characterize Pythium species from Punjab, Pakistan’s agricultural lands. Total of eleven Pythium (P. debaryanum, P. aphanidermatum, P. spinosum, P. irregulare, P. myriotylum, P. acanthicum, P. vexans, P. graminicola, P. dissotocum, P. echinulatum, and P. insidiosum) were isolated from the samples. Biometrics revealed the maximum values for aplerotic index were 59.8% for P. acanthicum and P. dissotocum while maximum values for ooplast index (39.6%) were calculated in the case of P. myriotylum and P. vexans. Molecular characterization, amplification of the ITS region revealed a genome size of approximately 570 bp. NCBI accession numbers for each species were acquired, and each species has shown 98–99% similarity with their reference sequences in the gene bank database. The research is a much-needed step to identify these pathogenic fungi morphologically and molecularly to understand its epidemiology and develop some potential management strategies.

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巴基斯坦旁遮普邦腐霉属植物的形态和分子特征

摘要腐霉属属于土生卵菌门。这项研究是为了鉴定巴基斯坦旁遮普邦农业用地的腐霉属物种。从样品中总共分离出11种腐霉（P.debaryanum、P.aphanidermatum、P.spinosum、P.irregular、P.myryotylum、P.acanthicum、P.vexans、P.graminicola、P.disstocum、P.echinulatum和P.insidiosum）。生物测定显示，棘皮P.acanthicum和溶解皮P.disstocum的再生指数最大值为59.8%，而肉豆蔻P.myryotylum和紫荆P.vexans的卵母细胞指数最大值（39.6%）。ITS区域的分子表征和扩增显示基因组大小约为570 bp。获得了每个物种的NCBI登录号，每个物种与基因库数据库中的参考序列显示出98–99%的相似性。这项研究是从形态学和分子生物学角度识别这些致病真菌以了解其流行病学并制定一些潜在管理策略的急需步骤。

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来源期刊

Archives of Phytopathology and Plant Protection Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.20

自引率

0.00%

发文量

100

期刊介绍： Archives of Phytopathology and Plant Protection publishes original papers and reviews covering all scientific aspects of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology, herbal studies and applied nematology and entomology as well as strategies and tactics of protecting crop plants and stocks of crop products against diseases. The journal provides a permanent forum for discussion of questions relating to the influence of plant protection measures on soil, water and air quality and on the fauna and flora, as well as to their interdependence in ecosystems of cultivated and neighbouring areas.