Protein adsorption on ZnO films studied by ATR-FTIR spectroscopy

IF 0.3 Q4 SPECTROSCOPY

Biomedical Spectroscopy and Imaging Pub Date : 2020-05-15 DOI:10.3233/bsi-200199

Wolfgang G. Hinze, M. Fallah, K. Hauser

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引用次数: 5

Abstract

. In many scientiﬁc ﬁelds there is a high interest to study molecular adsorption processes on surfaces. The adsorbed molecule can have signiﬁcant impact on the properties of the material under study, for example protein adsorption to inorganic material can enhance its biocompatibility. Attenuated total reﬂection Fourier-transform infrared (ATR-FTIR) spectroscopy is a suitable method to monitor such adsorption processes close to a surface. In this study, ZnO ﬁlms were synthesized on silicon ATR substrates via a mild hydrothermal reaction. The ﬁlms were then characterized by scanning electron microscopy (SEM) and FTIR microscopy. Chemical imaging with FTIR microscopy allowed to analyze the composition of the heterogeneous ﬁlm samples. ATR-FTIR spectroscopy was then applied to investigate the adsorption properties of the ZnO ﬁlms. Protein solutions of bovine serum albumine (BSA) were circulated in a closed cycle over the ZnO ﬁlm and IR spectra were recorded during the adsorption process. A stronger protein adsorption was observed for silicon substrates coated with ZnO than for plain silicon. Furthermore, subsequent ﬂushing with pure water and desorption measurements indicated a stronger protein binding to ZnO than to plain silicon.

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蛋白质在ZnO薄膜上吸附的ATR-FTIR光谱研究

.在许多科学领域，人们对研究表面分子吸附过程非常感兴趣。吸附的分子会对所研究材料的性能产生重大影响，例如蛋白质吸附到无机材料上可以增强其生物相容性。衰减全反射傅立叶变换红外光谱（ATR-FTIR）是监测靠近表面的这种吸附过程的合适方法。在本研究中，通过温和的水热反应在硅ATR衬底上合成了ZnO薄膜。然后通过扫描电子显微镜（SEM）和FTIR显微镜对薄膜进行表征。FTIR显微镜的化学成像可以分析异质薄膜样品的成分。然后应用ATR-FTIR光谱研究ZnO薄膜的吸附性能。牛血清白蛋白（BSA）的蛋白质溶液在ZnO薄膜上以封闭循环的方式循环，并在吸附过程中记录IR光谱。观察到用ZnO涂覆的硅衬底比普通硅更强的蛋白质吸附。此外，随后用纯水冲洗和解吸测量表明，与普通硅相比，蛋白质与ZnO的结合更强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedical Spectroscopy and Imaging SPECTROSCOPY-

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期刊介绍： Biomedical Spectroscopy and Imaging (BSI) is a multidisciplinary journal devoted to the timely publication of basic and applied research that uses spectroscopic and imaging techniques in different areas of life science including biology, biochemistry, biotechnology, bionanotechnology, environmental science, food science, pharmaceutical science, physiology and medicine. Scientists are encouraged to submit their work for publication in the form of original articles, brief communications, rapid communications, reviews and mini-reviews. Techniques covered include, but are not limited, to the following: • Vibrational Spectroscopy (Infrared, Raman, Teraherz) • Circular Dichroism Spectroscopy • Magnetic Resonance Spectroscopy (NMR, ESR) • UV-vis Spectroscopy • Mössbauer Spectroscopy • X-ray Spectroscopy (Absorption, Emission, Photoelectron, Fluorescence) • Neutron Spectroscopy • Mass Spectroscopy • Fluorescence Spectroscopy • X-ray and Neutron Scattering • Differential Scanning Calorimetry • Atomic Force Microscopy • Surface Plasmon Resonance • Magnetic Resonance Imaging • X-ray Imaging • Electron Imaging • Neutron Imaging • Raman Imaging • Infrared Imaging • Terahertz Imaging • Fluorescence Imaging • Near-infrared spectroscopy.