Taxonomic diversity and functional potential of microbial communities in salt lakes Gudzhirganskoe and Nukhe-Nur (Barguzin depression, Baikal Rift Zone)

Q3 Agricultural and Biological Sciences
E. Lavrentyeva, T. Banzaraktsaeva, V. Dambaev, L. Buyantueva, E. Valova, V. Ivanov, A. Plotnikov
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Abstract

On the territory of the Barguzin depression (Baikal Rift Zone) there are salt lakes, which are unique natural formations formed in exceptional natural conditions and experiencing extreme shifts in seasonal environmental conditions. This paper presents the results of the study of the microbial communities in the sulfate Lake Gudzhirganskoe and the soda Lake Nukhe-Nur in winter and summer seasons: from the assessment of the taxonomic composition to potential metabolic pathways. For the first time, based on the 16S rRNA gene sequencing data, the diversity of the microbial community of bottom sediments in summer and winter was studied. Environmental conditions such as pH, temperature and mineralization mainly determined the microbial community composition and led to noticeable shifts in the composition of the community at the level of families and genera. The change of the "summer" obligate and moderately alkalphilic and halophilic community to the "winter" alkali-, halotolerant/halophilic microbial community is observed in the winter period in Lake Gudzhirganskoe. In Lake Nukhe-Nur, a change from mesophilic-thermophilic community in summer to mesophilic-psychrophilic microbial community in winter was revealed. The totality of the obtained results gives an idea about the main trends in the seasonal dynamics of extremophilic microbial communities in the studied lakes in response to fluctuations in environmental parameters. The prediction of the metabolic pathways of prokaryotic communities using the Tax4Fun program made it possible to reveal similarities and differences in the metabolic potential of the microbial communities of the studied lakes. Potential functional genes have been found for all stages of the C, N and S cycles, with the exception of nitrification and aerobic CH4 oxidation.Only small qualitative and quantitative variations in the relative abundance of predicted functional genes were found between the microbial communities of the studied lakes. We predicted metabolic pathways that play an important role in the adaptation of microorganisms to changing environmental conditions. In general, it has been shown that there is a change in the dominant taxa at the level of families and genera in the microbial community in the summer and winter seasons, however, the predicted functional potential of microbial communities differed slightly by season and between the studied lakes.
古日尔甘斯克和努克-努尔盐湖微生物群落的分类多样性及功能潜力
在Barguzin凹陷(贝加尔湖裂谷带)的领土上有盐湖,这是在特殊的自然条件下形成的独特的自然形成,并经历着季节性环境条件的极端变化。本文介绍了对硫酸盐湖Gudzhirganskoe和苏打湖Nukhe Nur冬季和夏季微生物群落的研究结果:从分类组成的评估到潜在的代谢途径。首次基于16S rRNA基因测序数据,研究了夏季和冬季底质沉积物微生物群落的多样性。pH、温度和矿化等环境条件主要决定了微生物群落的组成,并导致群落组成在科属水平上发生显著变化。在Gudzhirganskoe湖的冬季,观察到“夏季”专性和中等嗜碱和嗜盐微生物群落向“冬季”耐碱、耐盐/嗜盐微生物种群的变化。Nukhe Nur湖从夏季的中温嗜热微生物群落转变为冬季的中温冷微生物群落。所获得的结果总体上说明了所研究湖泊中极端微生物群落对环境参数波动的季节动态的主要趋势。使用Tax4Fun程序预测原核群落的代谢途径,可以揭示所研究湖泊微生物群落代谢潜力的相似性和差异性。除了硝化作用和好氧CH4氧化作用外,在C、N和S循环的所有阶段都发现了潜在的功能基因。在所研究的湖泊的微生物群落之间,预测功能基因的相对丰度只有很小的定性和定量变化。我们预测了在微生物适应不断变化的环境条件中发挥重要作用的代谢途径。总的来说,研究表明,在夏季和冬季,微生物群落的科属水平上的优势类群发生了变化,然而,微生物群落预测的功能潜力在不同季节和所研究的湖泊之间略有不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biological Communications
Biological Communications Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.70
自引率
0.00%
发文量
21
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