Investigation of DNAmethylation of TWIST Gene in Breast Cancer and Its Relationship to Histopathological Features

乳腺癌(英文) Pub Date : 2019-01-23 DOI:10.4236/ABCR.2019.81004

Onur Eroglu, O. Çilingir, S. Artan, B. D. Aras

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引用次数: 1

Abstract

BACKGROUND: Promoter hypermethylation and global hypomethylation in the human genome are hallmarks of most cancers. OBJECTIVE: The aim of this study is to assess the methylation profile patterns of TWIST gene and to investigate the relationship of methylation with pathological features. METHODS: Romoter CpGisland methylation of TWIST which can be related in breast cancer was performed by methylation-sensitive high resolution melting (MS-HRM) analysis. Formalin-fixed, paraffin-embedded (FFPE) tissue samples of 80 patients with a diagnosis of primary breast cancer from Eskisehir Osmangazi University medical faculty of Oncology Clinic were included. RESULTS: In our study, the promoter hypermethylation frequency of TWIST gene was 25.0%. With these results, when the prognostic factors of the patients were analyzed, tumor stage and age were found to be meaningless with the hypermethylation of TWIST gene, but found to be significant with lymph node positivity, ER positivity, PR negativity, and HER2/NEU negativity. CONCLUSION: Our study is important as being the first study that analyzes association between histopathologic type and TWIST gene promotor methylation status in Turkish population.

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乳腺癌TWIST基因脱氧甲基化及其与组织病理特征关系的研究

背景:人类基因组中的启动子超甲基化和整体低甲基化是大多数癌症的标志。目的:研究TWIST基因的甲基化谱，探讨其与病理特征的关系。方法:采用甲基化敏感的高分辨率熔融(MS-HRM)分析方法对可能与乳腺癌相关的TWIST的母链CpGisland甲基化进行检测。本文收集了Eskisehir Osmangazi大学肿瘤诊所医学院诊断为原发性乳腺癌的80例经福尔马林固定、石蜡包埋(FFPE)组织样本。结果:本研究中，TWIST基因启动子超甲基化频率为25.0%。因此，在分析患者预后因素时，发现TWIST基因高甲基化对肿瘤分期和年龄无显著影响，而淋巴结阳性、ER阳性、PR阴性、HER2/NEU阴性对肿瘤分期和年龄有显著影响。结论:我们的研究是第一个分析土耳其人群中组织病理类型和TWIST基因启动子甲基化状态之间关系的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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乳腺癌(英文)

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