Sublingual immunotherapy by Nanogold in mice model of asthma

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Omid Javanmardghooghan, Fatemeh Azmoudeh, Mohammadamin Sadeghdoust, Farnaz Aligolighasemabadi, M. Khakzad
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Abstract

Objective(s): It has been shown that Nanogold particles have anti-inflammatory effects in different Rheumatologic, neurologic and gastrointestinal disease. They inhibit the synthesis of pro-inflammatory cytokines and also infiltration of inflammatory cells. Sublingual immunotherapy is a well-known effective, safe and clinically effective method way of immune response regulation which results in long-lasting symptoms reduction. This research was designed to find the immunological effects of sublingual immunotherapy using Nanogold in mice model of asthma. Materials and Methods: Twenty BALB/c mice were divided into four groups including one group of non-sensitized mice and three groups of asthmatic mice which were treated sublingually with PBS, Nanogold and Beclomethasone. IL-4 and IFN-γ levels were measured in serum and spleen cells supernatant using ELISA. BAL fluid inflammatory cells differential counting and lungs histological analysis were also done.Results: The results revealed that there was significant increase in level of IFN-γ and decrease in level of IL-4 in serum and spleen cells supernatant of Nanogold treated group (p <0.05). These findings indicates the shift of Th2/Th1 balance towards Th1 cells which is protective against asthma. In addition, histological and BAL fluid analysis demonstrated the reduction of cells and eosinophilic infiltration. Conclusion: Based on our results, sublingual immunotherapy by Nanogold has significant anti-inflammatory roll in asthmatic mice. Thus, Nanogold is a potentially valuable agent for controlling the underlying inflammation in asthma. However, further investigations is recommended to find more details about its effects.
纳米金舌下免疫治疗哮喘小鼠模型
目的:研究表明纳米金颗粒对不同的风湿病、神经系统疾病和胃肠道疾病具有抗炎作用。它们抑制促炎细胞因子的合成和炎症细胞的浸润。舌下免疫治疗是一种众所周知的有效、安全、临床有效的免疫反应调节方法,可持久减轻症状。本研究旨在观察纳米金舌下免疫治疗对哮喘小鼠模型的免疫效果。材料与方法:将20只BALB/c小鼠分为4组,其中1组为非致敏小鼠,3组为哮喘小鼠,分别用PBS、纳米金和倍氯米松舌下治疗。ELISA法检测血清和脾细胞上清中IL-4和IFN-γ水平。BAL液炎性细胞鉴别计数及肺组织分析。结果:纳米金处理组大鼠血清和脾细胞上清液中IFN-γ水平显著升高,IL-4水平显著降低(p <0.05)。这些发现表明Th2/Th1平衡向Th1细胞转移,这对哮喘有保护作用。此外,组织学和BAL液分析显示细胞和嗜酸性粒细胞浸润减少。结论:纳米金舌下免疫治疗对哮喘小鼠有明显的抗炎作用。因此,纳米金是一种潜在的有价值的药物,可以控制哮喘的潜在炎症。然而,建议进行进一步调查,以找到有关其影响的更多细节。
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
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