Association of 8p22-23-rs2254546 Genotypes with Coronary Artery Status and Resistance to Intravenous Immunoglobulin in Iranian Children with Kawasaki Disease

IF 0.5 Q4 PEDIATRICS

Archives of Pediatric Infectious Diseases Pub Date : 2021-02-14 DOI:10.5812/PEDINFECT.91586

Mehrnoush Hassas Yeganeh, Tolue Mahdavi, Alireza Firoozfar, Seyed Arman Saeedi, A. Shirvani, Ali Dehghanifard, Khosro Rhmani, M. Jari, Shabnam Hajian, R. Shiari

{"title":"Association of 8p22-23-rs2254546 Genotypes with Coronary Artery Status and Resistance to Intravenous Immunoglobulin in Iranian Children with Kawasaki Disease","authors":"Mehrnoush Hassas Yeganeh, Tolue Mahdavi, Alireza Firoozfar, Seyed Arman Saeedi, A. Shirvani, Ali Dehghanifard, Khosro Rhmani, M. Jari, Shabnam Hajian, R. Shiari","doi":"10.5812/PEDINFECT.91586","DOIUrl":null,"url":null,"abstract":"Background: Kawasaki disease (KD) is a frequent cause of acquired heart disease in infants and young children, which may be triggered by infectious agents, with the highest incidence in Asian countries. Several genotypes are identified as susceptibility genes for KD, but none is known in the Iranian population. Objectives: Due to the racial differences in the frequency and significance of the identified genes, we aimed to investigate the 8p22-23-rs2254546 genotypes in a sample of Iranian children and their association with aneurysm and resistance to treatment. Methods: In this prospective cross-sectional study, 100 children with KD as the sample group were compared with 100 matched unrelated healthy Iranian children with no history of KD or immune-related disease. The control subjects were ethnically recruited from the same hospital at the time of a routine physical examination. The 8p22-23-rs2254546 genotypes were analyzed using the polymerase chain reaction (PCR) and direct sequencing techniques, and the frequency of the three genotypes (GA, AA, and GG) was reported. Finally, the frequency of aneurysm and IVIG resistance was reported, and their associations with 8p22-23-rs2254546 genotypes were tested by SPSS software. Results: Of 100 patients with a mean age of 1.9 ± 1.7 (0.1 - 10.2) years, 57% were boys and 43% were girls. Of them, 62% had GG, 30% GA, and 8% AA genotypes. Besides, 21% of the studied patients were resistant to IVIG, and 19% had aneurysm, but resistance to IVIG and aneurysm was not associated with GG and GA genotypes of 8p22-23-rs2254546 (P = 0.29 and 0.88, respectively). Conclusions: The majority of the children with KD were shown to have this genetic susceptibility, which shows the importance of the 8p22-23-rs2254546 genotype in Iranian children. However, this genotype was not associated with the risk of resistance to IVIG and aneurysm.","PeriodicalId":44261,"journal":{"name":"Archives of Pediatric Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2021-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Pediatric Infectious Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5812/PEDINFECT.91586","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PEDIATRICS","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Kawasaki disease (KD) is a frequent cause of acquired heart disease in infants and young children, which may be triggered by infectious agents, with the highest incidence in Asian countries. Several genotypes are identified as susceptibility genes for KD, but none is known in the Iranian population. Objectives: Due to the racial differences in the frequency and significance of the identified genes, we aimed to investigate the 8p22-23-rs2254546 genotypes in a sample of Iranian children and their association with aneurysm and resistance to treatment. Methods: In this prospective cross-sectional study, 100 children with KD as the sample group were compared with 100 matched unrelated healthy Iranian children with no history of KD or immune-related disease. The control subjects were ethnically recruited from the same hospital at the time of a routine physical examination. The 8p22-23-rs2254546 genotypes were analyzed using the polymerase chain reaction (PCR) and direct sequencing techniques, and the frequency of the three genotypes (GA, AA, and GG) was reported. Finally, the frequency of aneurysm and IVIG resistance was reported, and their associations with 8p22-23-rs2254546 genotypes were tested by SPSS software. Results: Of 100 patients with a mean age of 1.9 ± 1.7 (0.1 - 10.2) years, 57% were boys and 43% were girls. Of them, 62% had GG, 30% GA, and 8% AA genotypes. Besides, 21% of the studied patients were resistant to IVIG, and 19% had aneurysm, but resistance to IVIG and aneurysm was not associated with GG and GA genotypes of 8p22-23-rs2254546 (P = 0.29 and 0.88, respectively). Conclusions: The majority of the children with KD were shown to have this genetic susceptibility, which shows the importance of the 8p22-23-rs2254546 genotype in Iranian children. However, this genotype was not associated with the risk of resistance to IVIG and aneurysm.

查看原文本刊更多论文

伊朗川崎病患儿8p223-rs2254546基因型与冠状动脉状况及静脉注射免疫球蛋白耐药性的关系

背景:川崎病(Kawasaki disease, KD)是婴幼儿获得性心脏病的常见病因，可能由感染性病原体引发，在亚洲国家发病率最高。几种基因型被确定为KD的易感基因，但在伊朗人群中没有已知基因型。目的:由于所鉴定基因的频率和意义存在种族差异，我们旨在研究伊朗儿童样本中的8p22-23-rs2254546基因型及其与动脉瘤和治疗耐药性的关系。方法:在这项前瞻性横断面研究中，将100名患有KD的儿童作为样本组，与100名无KD病史或免疫相关疾病的无血缘关系的健康伊朗儿童进行比较。对照受试者在常规体检时从同一家医院招募。采用聚合酶链反应(PCR)和直接测序技术对8p22-23-rs2254546基因型进行分析，并报道了GA、AA和GG三种基因型的频率。最后，报告动脉瘤发生频率和IVIG耐药性，并通过SPSS软件检验其与8p22-23-rs2254546基因型的相关性。结果:100例患者平均年龄1.9±1.7(0.1 ~ 10.2)岁，男孩占57%，女孩占43%。其中GG基因型62%，GA基因型30%，AA基因型8%。此外，21%的患者对IVIG有耐药性，19%的患者有动脉瘤，但对IVIG和动脉瘤的耐药性与8p22-23-rs2254546的GG和GA基因型无关(P分别= 0.29和0.88)。结论:大多数KD患儿具有这种遗传易感性，说明8p22-23-rs2254546基因型在伊朗患儿中的重要性。然而，该基因型与IVIG和动脉瘤抵抗风险无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Archives of Pediatric Infectious Diseases PEDIATRICS-

CiteScore

1.80

自引率

14.30%

发文量

期刊介绍： Archives Of Pediatric Infectious Disease is a clinical journal which is informative to all practitioners like pediatric infectious disease specialists and internists. This authoritative clinical journal was founded by Professor Abdollah Karimi in 2012. The Journal context is devoted to the particular compilation of the latest worldwide and interdisciplinary approach and findings including original manuscripts, meta-analyses and reviews, health economic papers, debates and consensus statements of clinical relevance to pediatric disease field, especially infectious diseases. In addition, consensus evidential reports not only highlight the new observations, original research and results accompanied by innovative treatments and all the other relevant topics but also include highlighting disease mechanisms or important clinical observations and letters on articles published in the journal.