Paul D. Riggs
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引用次数: 6
Abstract
Escherichia coli is often the first host chosen for recombinant protein production, due to its low cost, ease of use, and the wide range of molecular tools available. After the gene of interest is cloned, the first task of the researcher is to choose a suitable protein expression vector for protein production. The number of possible vectors is vast, but a good background of the advantages and disadvantages of the various types of vectors can help in designing a successful strategy. This unit provides an introduction to the elements that make up an expression vector and guidance in applying those elements to the production of a particular protein. © 2018 by John Wiley & Sons, Inc.
大肠杆菌蛋白表达载体综述
大肠杆菌通常是重组蛋白生产的首选宿主,因为它成本低,易于使用,并且有广泛的分子工具可用。克隆目标基因后,研究人员的第一个任务是选择合适的蛋白表达载体进行蛋白生产。可能的向量的数量是巨大的,但是对各种类型向量的优点和缺点的良好背景可以帮助设计一个成功的策略。本单元介绍了组成表达载体的元素,并指导将这些元素应用于特定蛋白质的生产。©2018 by John Wiley & Sons, Inc。
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