Novel time-resolved CARS implementation for application in microscopy

IF 1.9 4区物理与天体物理 Q3 OPTICS

Journal of the European Optical Society-Rapid Publications Pub Date : 2023-03-03 DOI:10.1051/jeos/2023008

P. Neethling

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引用次数: 0

Abstract

Vibrational dephasing times for benzene and carbon disulfide are measured using a custom single-beam Coherent Anti-stokes Raman Spectroscopy (CARS) setup. A femtosecond oscillator is used to pump a Polarization Maintaining All Normal Dispersion Photonic Crystal Fibre (PM-ANDi-PCF) to generate a broad band supercontinuum, covering a spectral region from 680 – 900 nm. The dispersion properties of the PM-ANDi-PCF ensures the supercontinuum is stable and there exists a fixed phase relationship between the spectral components of the supercontinuum. This enables its temporal compression using i2PIE, implemented using a liquid crystal spatial light modulator (SLM) in a 4f geometry. This SLM is also used to shape the pulse spectrally and temporally. With this setup we could demonstrate time-resolved CARS, measuring the vibrational relaxation times of a CS2/benzene mixture, and eliminate the non-resonant background completely. The main advantage of this setup is the fact that it is a single beam technique, eliminating the requirement for aligning the overlap of the pump and probe, both spatially and temporally, in the focal plane of the microscope. The strengths and limitations of the technique are highlighted and the route to time-resolved/background free vibrational microscopy is proposed.

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用于显微镜的新型时间分辨CARS实现

使用定制的单光束相干反斯托克斯拉曼光谱（CARS）装置测量苯和二硫化碳的振动退相时间。飞秒振荡器用于泵浦保偏全正常色散光子晶体光纤（PM ANDi PCF），以产生覆盖680–900 nm光谱区域的宽带超连续谱。PM ANDi PCF的色散特性确保了超连续谱是稳定的，并且在超连续谱的光谱分量之间存在固定的相位关系。这使得能够使用i2PIE对其进行时间压缩，i2PIE使用4f几何形状的液晶空间光调制器（SLM）来实现。该SLM还用于在频谱和时间上对脉冲进行整形。通过这种设置，我们可以演示时间分辨CARS，测量CS2/苯混合物的振动弛豫时间，并完全消除非共振背景。这种设置的主要优点是，它是一种单光束技术，消除了在显微镜焦平面上在空间和时间上对准泵浦和探针重叠的要求。强调了该技术的优势和局限性，并提出了实现时间分辨/无背景振动显微镜的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the European Optical Society-Rapid Publications 物理-光学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

5 weeks

期刊介绍： Rapid progress in optics and photonics has broadened its application enormously into many branches, including information and communication technology, security, sensing, bio- and medical sciences, healthcare and chemistry. Recent achievements in other sciences have allowed continual discovery of new natural mysteries and formulation of challenging goals for optics that require further development of modern concepts and running fundamental research. The Journal of the European Optical Society – Rapid Publications (JEOS:RP) aims to tackle all of the aforementioned points in the form of prompt, scientific, high-quality communications that report on the latest findings. It presents emerging technologies and outlining strategic goals in optics and photonics. The journal covers both fundamental and applied topics, including but not limited to: Classical and quantum optics Light/matter interaction Optical communication Micro- and nanooptics Nonlinear optical phenomena Optical materials Optical metrology Optical spectroscopy Colour research Nano and metamaterials Modern photonics technology Optical engineering, design and instrumentation Optical applications in bio-physics and medicine Interdisciplinary fields using photonics, such as in energy, climate change and cultural heritage The journal aims to provide readers with recent and important achievements in optics/photonics and, as its name suggests, it strives for the shortest possible publication time.