Inoculación con rizobios y hongos micorrízicos arbusculares en plantas de Leucaena leucocephala en etapa de vivero y en sustrato con pH neutro

IF 0.9 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Tropical Grasslands-Forrajes Tropicales Pub Date : 2022-05-31 DOI:10.17138/tgft(10)98-108

Gustavo Crespo-Flores, Hugo M. Ramírez-Tobias, M. R. Vallejo-Pérez, H. Méndez-Cortés, Pedro J. González-Cañizares

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引用次数: 1

Abstract

The objective of this work was to evaluate the effect of the simple and combined inoculation with three local rhizobia isolates (R1, R2 and R3) and two species of arbuscular mycorrhizal fungi (Glomus cubense -AMF1- and Claroideoglomus claroideum -AMF2-) on mycorrhizal colonization, nodulation, growth and biomass production of Leucaena leucocephala in a substrate with a close to neutrality pH under greenhouse conditions. Thirteen treatments were evaluated in a completely randomized design with five replications. The inoculation and co-inoculation promoted greater plant growth with respect to the control without inoculation and to the fertilization treatment. Within the inoculation and co-inoculation treatments, one rhizobium isolate (R2) stood out for producing the highest plant height and leaflet production, while the R3 + AMF1 and R3 + AMF2 combinations promoted the highest plant height, and also promoted higher biomass together with R1 + AMF1. In addition, the R3 + AMF2 combination stood out for presenting a high number of AMF spores, frequency and intensity by AMF colonization, and nodule activity. It is concluded that inoculation with local rhizobia isolates and their combination with AMF favors the development of mycorrhizal structures, nodulation, growth and biomass production of L. leucocephala grown in a substrate with neutral pH. Isolate R2 and the combination R3 + AMF2 were identified as effective inoculants to increase plant growth.

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在苗圃阶段和中性pH基质中接种银合欢植物的根瘤菌和丛枝菌根真菌

本工作的目的是评估三个当地根瘤菌分离株（R1、R2和R3）和两种丛枝菌根真菌（Glomus cubense-AMF1-和Claroideoglomus claroideum-AMF2-）的简单联合接种对菌根定殖、结瘤，在温室条件下，银合欢在接近中性pH的基质中的生长和生物量生产。在一个完全随机的设计中对13种治疗方法进行了评估，共进行了5次重复。与未接种的对照和施肥处理相比，接种和共接种促进了更大的植物生长。在接种和共接种处理中，一个根瘤菌分离株（R2）的株高和小叶产量最高，而R3+AMF1和R3+AMF2组合的株高最高，并且与R1+AMF1一起提高了生物量。此外，R3+AMF2组合因呈现大量AMF孢子、AMF定殖的频率和强度以及根瘤活性而脱颖而出。结果表明，接种当地根瘤菌分离株及其与AMF的组合有利于在中性pH的基质中生长的白头翁菌根结构的发育、结瘤、生长和生物量的产生。分离株R2和组合R3+AMF2是提高植物生长的有效接种剂。

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来源期刊

Tropical Grasslands-Forrajes Tropicales Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： The Journal publishes, in English or Spanish, Research Papers and Short Communications on research and development, as well as contributions from practitioners (Farmer Contributions) and Review Articles, related to pastures and forages in the tropics and subtropics. There is no regional focus; the information published should be of interest to a wide readership, encomprising researchers, academics, students, technicians, development workers and farmers. In general, the focus of the Journal is more on sown (''improved'') pastures and forages than on rangeland-specific aspects of natural grasslands, but exceptions are possible (e.g. when a submission is relevant for a particularly broad readership in the pasture and forage science community). The Journal will also consider the occasional publication of associated, but closely related, research in the form of an additional scientific communication platform [e.g. a re-make of the former Genetic Resources Communication series of the former Division of Tropical Crops and Pastures of the Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia]. Areas of particular interest to the Journal are: Forage Genetic Resources and Livestock Production[...] Environmental Functions of Forages[...] Socio-economic Aspects[...] Topics within the aforementioned areas may include: Diversity evaluation; Agronomy; Establishment (including fertilization); Management and utilization; Animal production; Nutritive value; Biotic stresses (pests and diseases, weeds); Abiotic stresses (soil fertility, water, temperature); Genetics and breeding; Biogeography and germplasm collections; Seed production; Ecology; Physiology; Rhizobiology (including BNF, BNI, mycorrhizae); Forage conservation; Economics; Multilocational experimentation; Modelling.