Seroprevalence of bacterial isolates and their antimicrobial susceptibility pattern from exudate sample in Birla Institute of Medical Research Hospital, Gwalior, India

Kamini Rajput, S. Johri, A. Goyal
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Abstract

Background and Aim: Infection characterized by the production of pus is classified as pyogenic or pus-producing infections. These infections are challenging to treat because bacteria are becoming increasingly resistant to antibiotics. It is critical to understand the microorganisms that cause infections and their antibiotic susceptibility patterns for optimal management of the patient. The purpose of this study was to examine the frequency and distribution of bacteria isolated from pus/wound, as well as their susceptibility patterns. Materials and Methods: A retrospective hospital-based study was conducted on 175 patients who provided pus and/or wound discharge samples in different wards (outpatient department or inpatient department). The samples were inoculated on MacConkey agar and blood agar plates and incubated at 37°C for 24 h. The Gram stain and biochemical tests were used to identify all isolates after incubation. Antibiotic sensitivity was performed using Kirby–Bauer disc diffusion test. Results: This study covered 175 patients, with a bacterial isolation rate of 102 (58.28%). Males outnumbered females in the samples (M: F-1.8:1), with a median age of 45 years. Majority were in the age group of 40–60 years which was 41 (40.20%). Monomicrobial infections were seen in 92 (90.19%) samples whereas polymicrobial infections in 10 (9.80%) samples and total 112 bacterial strains were isolated. Conclusion: Escherichia coli was the most prevalent isolate in our investigation, followed by Pseudomonas aeruginosa. For Gram-positive cocci, doxycycline, linezolid, and chloramphenicol are the most effective antibiotics, whereas for Gram-negative bacilli, amikacin, gentamicin, and chloramphenicol are the most effective antibiotics. The susceptibility statistics presented in this article may be useful in formulating empiric treatment regimens for pyogenic infections.
印度瓜利尔Birla医学研究所医院渗出液中分离细菌的血清阳性率及其药敏模式
背景与目的:以产脓为特征的感染分为化脓性感染或产脓性感染。这些感染的治疗具有挑战性,因为细菌对抗生素的耐药性越来越强。了解引起感染的微生物及其抗生素敏感性模式对患者的最佳管理至关重要。本研究的目的是研究从脓液/伤口中分离的细菌的频率和分布,以及它们的易感模式。材料与方法:对175例在不同病房(门诊部或住院部)提供脓液和/或伤口分泌物样本的患者进行回顾性研究。将样品接种于MacConkey琼脂和血琼脂平板上,37℃孵育24 h。孵育后采用革兰氏染色法和生化试验对所有分离株进行鉴定。采用Kirby-Bauer圆盘扩散试验测定抗生素敏感性。结果:本研究共纳入175例患者,细菌分离率102例(58.28%)。样本中男性多于女性(M: f: 1.8:1),中位年龄为45岁。以40 ~ 60岁年龄组居多,41例(40.20%)。检出单菌感染92例(90.19%),多菌感染10例(9.80%),共检出112株细菌。结论:大肠杆菌是本次调查中最常见的分离菌,其次是铜绿假单胞菌。对于革兰氏阳性球菌,强力霉素、利奈唑胺和氯霉素是最有效的抗生素,而对于革兰氏阴性杆菌,阿米卡星、庆大霉素和氯霉素是最有效的抗生素。本文中提出的敏感性统计可能有助于制定化脓性感染的经验性治疗方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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