Effects of knockout of CO2-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice

IF 1.6 3区农林科学 Q2 AGRONOMY

Plant Production Science Pub Date : 2023-07-03 DOI:10.1080/1343943X.2023.2233161

Fumihiro Miyagawa, Naoki Shibatani, R. Morita, Daisuke Sasayama, T. Hatanaka, T. Azuma, H. Fukayama

引用次数: 0

Abstract

ABSTRACT CO2-Responsive CCT Protein (CRCT) is suggested to be a positive regulator of starch synthesis-related genes in the vegetative organs of rice. To investigate the physiological function of CRCT, we produced CRCT knockout lines by CRISPR/Cas9 system. CRCT knockout tended to slightly reduce photosynthetic performance and had some adverse effects on growth parameters such as tiller number and straw dry weight. The starch content in the leaf sheath of CRCT knockout lines was significantly lower than that of wild-type. qRT-PCR and RNA-seq analysis showed that sucrose treatment induced the expression of CRCT, which, in turn, induced many starch synthesis-related genes in wild-type, whereas this induction did not occur in CRCT knockout lines. In conclusion, our results suggest that sugar induction of starch synthesis-related genes is almost fully dependent on CRCT, making it a necessary factor for normal photosynthesis and growth of rice. GRAPHICAL ABSTRACT

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敲除淀粉合成调节剂CCT蛋白对水稻生理特性的影响

摘要CO2响应性CCT蛋白（CRCT）被认为是水稻营养器官中淀粉合成相关基因的正调控因子。为了研究CRCT的生理功能，我们利用CRISPR/Cas9系统制备了CRCT敲除株。CRCT敲除往往会略微降低光合性能，并对分蘖数和秸秆干重等生长参数产生一些不利影响。CRCT敲除系叶鞘中的淀粉含量显著低于野生型。qRT-PCR和RNA-seq分析表明，蔗糖处理诱导了CRCT的表达，进而在野生型中诱导了许多淀粉合成相关基因，而这种诱导在CRCT敲除系中没有发生。总之，我们的结果表明，淀粉合成相关基因的糖诱导几乎完全依赖于CRCT，使其成为水稻正常光合作用和生长的必要因素。图形摘要

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来源期刊

Plant Production Science 农林科学-农艺学

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

>36 weeks

期刊介绍： Plant Production Science publishes original research reports on field crops and resource plants, their production and related subjects, covering a wide range of sciences; physiology, biotechnology, morphology, ecology, cropping system, production technology and post harvest management. Studies on plant production with special attention to resource management and the environment are also welcome. Field surveys on cropping or farming system are also accepted. Articles with a background in other research areas such as soil science, meteorology, biometry, product process and plant protection will be accepted as long as they are significantly related to plant production.