Comet Assay: Quantification of Damaged DNA in Catla catla Exposed to Endosulfan+Chlorpyrifos

Abstract

| In an acute toxicity trail, LC50 and LC100value (96 hr) of endosulfan (END)+chlorpyrifos (CPF) mixture for Catla catla was computed. The genotoxicity of END+CPF mixture in RBCs of fish was also evaluated by comet assay. To check the genotoxicity of END+CPF mixture, blood of fish was sampled after 24-hr of intervals. Some fishes were also kept in clean water known as negative control (NC) and for positive control (PC) cyclophosphamide was injected into fish. The tolerance limits of C. catla against END+CPF was computed as 1.35±0.01μgL(LC50) and 2.25±0.02μgL(LC100). Genotoxic results showed that END+CPF mixture caused significant damage to nuclei (22.58±5.37%) and GDI (0.73±0.15%) in RBCs of fish as compare to control. Aduration-specific damage to DNA was observed in RBCs of C. catla. Article History Received: September 04, 2018 Revised: June 03, 2019 Accepted: June 08, 2019 Published: June 21, 2019 Authors’ Contributions HN performed the research and wrote the article. SA planned and supervised the study. KA provided lab facilities for research. MAT and LS helped in writing the article. GN helped in statistical anlyses.