Transcriptome analysis of a transgenic Arabidopsis plant overexpressing CsBCAT7 reveals the relationship between CsBCAT7 and branched-chain amino acid catabolism

Q3 Agricultural and Biological Sciences

Journal of Plant Biotechnology Pub Date : 2021-12-31 DOI:10.5010/jpb.2021.48.4.228

Young-Cheon Kim, Dong Sook Lee, Youjin Jung, Eun Bin Choi, Jungeun An, Sanghyeob Lee, Jong-Seop Lee

{"title":"Transcriptome analysis of a transgenic Arabidopsis plant overexpressing CsBCAT7 reveals the relationship between CsBCAT7 and branched-chain amino acid catabolism","authors":"Young-Cheon Kim, Dong Sook Lee, Youjin Jung, Eun Bin Choi, Jungeun An, Sanghyeob Lee, Jong-Seop Lee","doi":"10.5010/jpb.2021.48.4.228","DOIUrl":null,"url":null,"abstract":"The amino acids found in plants play important roles in protein biosynthesis, signaling processes, and stress responses, and as components in other biosynthesis pathways. Amino acid degradation helps maintain plant cells' energy states under certain carbon starvation conditions. Branched-chain amino acid transferases (BCATs) play an essential role in the metabolism of branched-chain amino acids (BCAAs) such as isoleucine, leucine and valine. In this paper, we performed genome-wide RNA-seq analysis using CsBCAT7 -overexpressing Arabidopsis plants. We observed significant changes in genes related to flowering time and genes that are germination-responsive in transgenic plants. RNA-seq and RT-qPCR analyses revealed that the expression levels of some BCAA catabolic genes were upregulated in these same transgenic plants, and that this correlated with a delay in their senescence phenotype when the plants were placed in extended darkness conditions. These results suggest a connection between BCAT and the genes implicated in BCAA catabolism.","PeriodicalId":16797,"journal":{"name":"Journal of Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5010/jpb.2021.48.4.228","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

Abstract

The amino acids found in plants play important roles in protein biosynthesis, signaling processes, and stress responses, and as components in other biosynthesis pathways. Amino acid degradation helps maintain plant cells' energy states under certain carbon starvation conditions. Branched-chain amino acid transferases (BCATs) play an essential role in the metabolism of branched-chain amino acids (BCAAs) such as isoleucine, leucine and valine. In this paper, we performed genome-wide RNA-seq analysis using CsBCAT7 -overexpressing Arabidopsis plants. We observed significant changes in genes related to flowering time and genes that are germination-responsive in transgenic plants. RNA-seq and RT-qPCR analyses revealed that the expression levels of some BCAA catabolic genes were upregulated in these same transgenic plants, and that this correlated with a delay in their senescence phenotype when the plants were placed in extended darkness conditions. These results suggest a connection between BCAT and the genes implicated in BCAA catabolism.

查看原文本刊更多论文

对过表达CsBCAT7转基因拟南芥植株的转录组分析揭示了CsBCAT7与支链氨基酸分解代谢之间的关系

在植物中发现的氨基酸在蛋白质生物合成、信号过程和胁迫反应中发挥重要作用，并作为其他生物合成途径的组成部分。氨基酸降解有助于维持植物细胞在某些碳饥饿条件下的能量状态。支链氨基酸转移酶(BCATs)在异亮氨酸、亮氨酸和缬氨酸等支链氨基酸(BCAAs)的代谢中起重要作用。在本文中，我们使用过表达CsBCAT7的拟南芥植物进行了全基因组RNA-seq分析。我们观察到转基因植物中与开花时间相关的基因和发芽响应基因发生了显著变化。RNA-seq和RT-qPCR分析显示，在这些转基因植物中，一些BCAA分解代谢基因的表达水平上调，这与植物在长时间黑暗条件下延迟衰老表型有关。这些结果表明BCAT与BCAA分解代谢相关基因之间存在联系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Plant Biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Journal of Plant Biotechnology (JPB) is an international open access journal published four issues of a yearly volume on March 31, June 30, September 30, and December 31 by The Korean Society for Plant Biotechnology (KSPBT) founded in 1973. JPB publishes original, peer-reviewed articles dealing with advanced scientific aspects of plant biotechnology, which includes molecular biology, genetics, genomics, proteomics, and metabolomics. JPB does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.