Phytochemical Screening, In Vitro Antidiabetic and Antioxidant Activity of Rabdosia rugosa (Wall. ex Benth.) H. Hara Extract from Kinnaur District, Himachal Pradesh

Abstract

Rabdosia rugosa (Wall. ex Benth.) H. Hara. (Syn. Plectranthusrugosus Wall.) belongs to the Lamiaceae family and is used by local communities of the Kinnaur district to cure diabetes. The selection of the plant material is based upon data that aerial parts of the plant (leaves along with flowers) are used in folklore to manage the postprandial hike in blood sugar levels. Therefore, in the present investigation, antiradical and antidiabetic activities of the ethanolic extract of the plant were checked. Preliminary tests for phytochemicals, total phenols, and flavenoids were also determined. Our results manifested that R. rugosa (Rabdosia rugosa) extract exhibited superior antioxidant activity (p˂0.05) by DPPH and H2O2 assays with IC50 = 9.62 ± 2.70 μg/ml and IC50 = 28.22 ± 2.94 μg/ml. This plant also exhibited preeminent repression action against the enzyme, i.e., alpha-amylase and alpha-glucosidase (p˂0.05) with IC50 = 10.49 ± 6.17 μg/ml (Porcine α – amylase), IC50 = 1.87 ± 1.79 μg/ml (Yeast α - glucosidase), ( IC50 = 16.89 ± 0. 06 μg/ml(Intestinal rat α - glucosidase) Hence, we concluded that present plant possessed excellent antiradical and antidiabetic activity. The antidiabetic activity was estimated higher than the standard Acarbose. As per our information, this may be the first time to report on the extract of R. rugosa for its antioxidant and antidiabetic capacity. The current findings divulge that this plant can further be studied to verify its therapeutic activities.