Metabolomic profiling upon external digestion in larvae of diving beetles: Cybister Curtis, 1827, Dytiscus Linnaeus, 1758, and Hydaticus Leach, 1817 (Coleoptera: Dytiscidae)

IF 0.5 4区农林科学 Q4 ENTOMOLOGY

Aquatic Insects Pub Date : 2022-09-21 DOI:10.1080/01650424.2022.2076883

T. Inoda, S. Kamimura

引用次数: 0

Abstract

Abstract The SDS–PAGE analysis of prey animals (fly larvae) during external digestion by aquatic predators, the larvae of selected species in dytiscid genera, showed a major component of protein with a molecular weight of ca. 20 kDa. In addition, the analysis of third instar larvae of Cybister brevis Aubé, 1838, by nanoLC–ESI–Q-TOF/MS/MS revealed that the digested body fluid includes a polypeptide with a sequence of 97 amino acids corresponding to hemocyanin N (51% matched by Mascot and BLAST searches) and hemocyanin M (12% matched) derived from flies. We also found evidence indicating that beetle larvae repeatedly release digestive enzymes at least twice while consuming the fly bodies. These results suggest that the digested polypeptides were derived from ubiquitous high molecular substances such as arylphorin subunit C223 precursor included in the body fluid of the fly (Calliphora vicina Robineau-Desvoidy, 1830) that were produced during external digestion by diving beetle larvae.

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潜水甲虫幼虫体外消化的代谢组学分析：Cybister Curtis，1827，林奈Dytiscus Linnaeus，1758，和Hydraticus Leach，1817（鞘翅目：Dytiscidae）

摘要在水生捕食者（dytiscid属中选定物种的幼虫）的外部消化过程中，对猎物（苍蝇幼虫）的SDS–PAGE分析显示，蛋白质的主要成分分子量约为20 kDa。此外，通过nanoLC–ESI–Q-TOF/MS/MS对Cybister brevis Aubé，1838的三龄幼虫进行的分析表明，消化后的体液包括一种具有97个氨基酸序列的多肽，对应于来自苍蝇的血蓝蛋白N（51%通过Mascot和BLAST搜索匹配）和血蓝蛋白M（12%匹配）。我们还发现证据表明，甲虫幼虫在吞噬苍蝇身体时至少两次重复释放消化酶。这些结果表明，消化的多肽来源于普遍存在的高分子物质，如蝇体液中包含的芳基蛋白亚基C223前体（Calliphora vicina Robineau Desvoidy，1830），这些物质是由潜水甲虫幼虫在外部消化过程中产生的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Insects 生物-昆虫学

CiteScore

2.00

自引率

25.00%

发文量

审稿时长

>12 weeks

期刊介绍： Aquatic Insects is an international journal publishing original research on the systematics, biology, and ecology of aquatic and semi-aquatic insects. The subject of the research is aquatic and semi-aquatic insects, comprising taxa of four primary orders, the Ephemeroptera, Odonata, Plecoptera, and Trichoptera but also aquatic and semi-aquatic families of Hemiptera, Coleoptera, and Diptera, as well as specific representatives of Hymenoptera , Lepidoptera, Mecoptera, Megaloptera , and Neuroptera that occur in lotic and lentic habitats during part of their life cycle. Studies on other aquatic Hexapoda (i.e., Collembola) will be only accepted if space permits. Papers on other aquatic Arthropoda (e.g., Crustacea) will not be considered, except for those closely related to aquatic and semi-aquatic insects (e.g., water mites as insect parasites). The topic of the research may include a wide range of biological fields. Taxonomic revisions and descriptions of individual species will be accepted especially if additional information is included on habitat preferences, species co-existing, behavior, phenology, collecting methods, etc., that are of general interest to an international readership. Descriptions based on single specimens are discouraged. Detailed studies on morphology, physiology, behavior, and phenology of aquatic insects in all stadia of their life cycle are welcome as well as the papers with molecular and phylogenetic analyses, especially if they discuss evolutionary processes of the biological, ecological, and faunistic formation of the group.