Incidence of Chryseobacterium spp. in raw milk and some dairy products

A. S. Mohamed, L. El-Malt, Karima G. Abdel Hameed, Mona A El-Zamkan
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Abstract

A total of 225 random samples of raw cow milk (150), Kareish cheese, Domiati cheese and butter (25 samples each), were collected from various farmers’ houses, dairy shops, street vendors and supermarkets located at Aswan city, Egypt, to be examined by culture and molecular methods in which 16S rRNA gene was used; the obtained PCR product was then purified and sequenced to detect the existence of Chryseobacterium spp. All cheese and butter samples didn’t show the characteristic colonies of Chryseobacterium spp., while two raw cow milk samples exhibited the characteristic Chryseobacterium colonies. The suspected Chryseobacterium spp. were confirmed by PCR and DNA sequencing and comparing the sequenced DNA with those on GenBank using the BLAST tool on GenBank, underlining its occurrence in raw cow milk samples with an incidence of 1.3%. The isolated strains showed the highest similarity with Chryseobacterium jip 108/83 (100%). According to obtained data and our knowledge, it is the first time to isolate Chryseobacterium spp. from raw milk in Egypt. The isolated strains were registered on the GenBank with the proposed names Chryseobacterium Egyam strain AM1 (Accession No. MK059947) and Chryseobacterium Egyam strain AM2 (Accession No. MK059948). A constructed phylogenetic tree by using the neighbour-joining method was applied to detect the exact taxonomic position of the obtained strains.
原料奶及部分乳制品中黄杆菌的发病率
从埃及阿斯旺市的农民住宅、乳制品店、街头小贩和超市中随机抽取生牛奶(150份)、Kareish奶酪、Domiati奶酪和黄油(各25份)共225份样本,采用培养和使用16S rRNA基因的分子方法进行检测;对所得PCR产物进行纯化和测序,发现所有奶酪和黄油样品均未显示出黄杆菌的特征菌落,而两种原料牛奶样品显示出黄杆菌的特征菌落。经PCR和DNA测序,并利用GenBank上的BLAST工具与GenBank上的DNA进行比对,证实该菌株存在于原牛奶样品中,发生率为1.3%。分离菌株与黄杆菌jip 108/83相似度最高(100%)。根据已获得的资料和我们的知识,这是埃及首次从原料奶中分离到黄杆菌。分离得到的菌株已在GenBank上注册,命名为埃及黄杆菌AM1菌株(登录号:AM1)。MK059947)和埃及黄杆菌菌株AM2(注册号:MK059948)。采用邻域连接法构建系统发育树,对得到的菌株进行准确的分类定位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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