Development of mucoadhesive thiomeric chitosan nanoparticles for the targeted ocular delivery of vancomycin against Staphylococcus aureus resistant strains

IF 3.3 Q3 NANOSCIENCE & NANOTECHNOLOGY

Nanofabrication Pub Date : 2020-01-01 DOI:10.1515/nanofab-2020-0105

Faryal Jahan, S. Zaman, Sohail Akhtar, R. Arshad, Ibrahim M. Ibrahim, G. Shahnaz, A. Rahdar, S. Pandey

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引用次数: 6

Abstract

Abstract This study aims to formulate mucoadhesive vancomycin loaded thiolated chitosan (TCS) nanoparticles. These nanoparticles are mucoadhesive and enhance the retention of the drug at the ocular site. For this purpose, TCS loaded vancomycin nanoparticles were prepared by the ion-gelation method and were characterized for their size, shape, polydispersity index, mucoadhesion, cellular uptake and anti-inflammatory activity. The average size of the synthesized nanoparticles was found to be 288 nm with positive zeta potential. Moreover, 85% vancomycin was successfully encapsulated in TCS nanoparticles by using this method. A 2-fold increase in mucoadhesion was found as compared to non-thiolated vancomycin formulation (p < 0.05). Zone of inhibition of vancomycin loaded TCS was also significantly improved compared to non-thiolated chitosan nanoparticles and vancomycin alone. In-vivo anti-inflammatory evaluation via histopathology resulted in ocular healing. Based on the results, it is inferred that TCS nanoparticles are a promising drug delivery carrier system for ocular delivery of vancomycin.

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黏附硫聚壳聚糖纳米颗粒用于万古霉素抗金黄色葡萄球菌耐药菌株的眼部靶向递送

摘要本研究旨在制备粘粘万古霉素负载的硫代壳聚糖（TCS）纳米粒子。这些纳米颗粒具有粘膜粘附性，可增强药物在眼部的滞留性。为此，通过离子凝胶法制备了TCS负载的万古霉素纳米颗粒，并对其大小、形状、多分散指数、粘膜粘附、细胞摄取和抗炎活性进行了表征。发现合成的纳米颗粒的平均尺寸为288nm，具有正ζ电位。此外，用该方法成功地将85%的万古霉素包埋在TCS纳米颗粒中。与非巯基化万古霉素制剂相比，粘膜粘附增加了2倍（p<0.05）。与非巯基壳聚糖纳米颗粒和单独的万古霉素相比，负载万古霉素的TCS的抑制区也显著改善。通过组织病理学进行体内抗炎评估，导致眼部愈合。基于这些结果，可以推断TCS纳米颗粒是一种很有前途的万古霉素眼部给药载体系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanofabrication NANOSCIENCE & NANOTECHNOLOGY-

自引率

10.30%

发文量

审稿时长

16 weeks