Th1 / Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows

IF 1.1 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Mljekarstvo Pub Date : 2022-03-30 DOI:10.15567/mljekarstvo.2022.0204

T. Safak

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引用次数: 2

Abstract

The aim of this study to determine the Th1/Th2 cytokine balance in milk according to the bacterial species that cause subclinical mastitis in cows. The California Mastitis Test (CMT) was applied to the selected cows. The cows were divided into four groups: cows with negative CMT (n = 45); Escherichia coli (E. coli) group included only cows with E. coli growing in CMT-positive milk samples (n = 45); Streptococcus agalactiae (S. agalactiae) group included cows with only S. agalactiae growing in CMT-positive milk samples (n = 45); Staphylococcus aureus (S. aureus) group included cows with only S. aureus growing in CMT-positive milk samples (n = 45). Somatic cell count (SCC) in fresh milk samples was measured using the DeLaval Cell Counter device. Also, cytokine analyses were performed using Species-specific commercial ELISA kits. Tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) concentrations were relatively high in the E. coli group, but the interleukin (IL)-2 concentration was low. The lowest concentration of IL-4 was found in the CMT-negative group. The highest IL-5 concentration was found in the S. agalactiae group, while the highest milk IL-10 concentration was found in the S. aureus group. Also, T helper (Th1/Th2) polarization shifted towards Th1 in milk with mastitis caused by E. coli. Th1/Th2 polarization was shifted to Th2 in milk with mastitis caused by S. aureus and S. agalactiae. Based on our findings, cellular immunity should be maintained in mastitis cases due to E. coli, and humoral immunity should be supported in mastitis caused by S. aureus and S. agalactiae.

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不同病原体引起奶牛亚临床乳腺炎的乳汁中Th1/Th2细胞因子极化

本研究的目的是根据导致奶牛亚临床乳腺炎的细菌种类来确定牛奶中Th1/Th2细胞因子的平衡。加利福尼亚乳腺炎测试（CMT）应用于选定的奶牛。将奶牛分为四组：CMT阴性奶牛（n=45）；大肠杆菌（E.coli）组仅包括CMT阳性牛奶样本中生长有大肠杆菌的奶牛（n=45）；无乳链球菌（无乳链球菌）组包括在CMT阳性牛奶样本中仅生长有无乳链球菌的奶牛（n=45）；金黄色葡萄球菌（S.aureus）组包括CMT阳性牛奶样本中仅生长有金黄色葡萄菌的奶牛（n=45）。使用利拉伐细胞计数装置测量新鲜牛奶样品中的体细胞计数（SCC）。此外，使用物种特异性商业ELISA试剂盒进行细胞因子分析。大肠杆菌组的肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）浓度相对较高，但白细胞介素（IL）-2浓度较低。CMT阴性组的IL-4浓度最低。无乳链球菌组的IL-5浓度最高，而乳IL-10浓度最高的是金黄色葡萄球菌组。此外，在由大肠杆菌引起的乳腺炎的牛奶中，辅助T细胞（Th1/Th2）极化向Th1转移。在由金黄色葡萄球菌和无乳链球菌引起的乳腺炎的乳汁中，Th1/Th2极化转变为Th2。根据我们的研究结果，大肠杆菌引起的乳腺炎应保持细胞免疫，金黄色葡萄球菌和无乳链球菌引起的乳房炎应支持体液免疫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mljekarstvo Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

1.90

自引率

41.70%

发文量

审稿时长

12 weeks

期刊介绍： Mljekarstvo is an open access, peer-reviewed international quarterly scientific journal. The first issue was published in 1951, by the Croatian Dairy Operators'' Association (today: Croatian Dairy Union, publisher). In a paper at a Union conference held 28 October 1951 in Zagreb it was said: "Our desire is that this magazine does not meet the fate of its predecessors, but that it continues to reflect the creative efforts and to provide guidelines for the producers as well as all other operators employed in the dairy industry." It is our pleasure today to say that wishes of the enthusiasts who attended the conference have come true, and the magazine Mljekarstvo during the last six decades was a reflection of the creative efforts of numerous dairy scientists and experts, and through its texts it served as a guideline in improving production and processing of milk and dairy products. Mljekarstvo has been following all the achievements of the dairy profession in Croatia, and it also gives the short surveys of world achievements. The result of the research of local and foreign scientists and experts always find their place in the magazine Mljekarstvo. It has been edited by our outstanding dairy experts employed at colleges, research institutions and dairy companies.