Effect of human amnion-derived multipotent progenitor cells on hematopoietic recovery after total body irradiation in C57BL/6 mice

Q4 Health Professions
Y. Du, R. Banas, Elizabeth A McCart, Jeffy George, K. Oakley, Y. Han, M. Landauer, R. Day
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引用次数: 3

Abstract

Background: The hematopoie c system is sensi ve to the adverse effects of ionizing radia on. Cellular therapies u lizing mesenchymal stem cells or vascular endothelial cells have been explored as poten al countermeasures for radia on hematopoie c injuries. We inves gated cells cultured from amnion (Amnion-derived Mul potent Progenitor cells, AMPs) for effects on hematopoie c recovery following total body irradia on in mice. Materials and Methods: C57BL/6J mice were sham-irradiated or exposed to 60 Co irradia on (7.75 – 7.90 Gy, 0.6 Gy/min). Either AMPs (5 × 10 6 cells/animal) or vehicle were administered 24 h pos rradia on via intraperitoneal injec on. Results: We observed a 13% and 20% improvement in 30-day survival of mice treated with AMPs compared with treatment with vehicle following irradia on at 7.75 and 7.90 Gy, respec vely. AMP treatment was characterized by a trend toward accelerated recovery of white blood cells, neutrophils, re culocytes, and monocytes, measured through day 40 pos rradia on a9er 7.75 Gy. AMP treatment enhanced hematopoie c cell repopula on of spleen and femoral bone marrow as measured by total nucleated cell and hematopoie c progenitor cell counts in comparison to vehicle-treated animals. FACS analysis showed that AMPs treatment significantly mi gated the reduc on in CD11b + /Gr-1 int and CD11b + /Gr-1 high bone marrow cell popula ons at the nadir, and improved recovery of these cell types. Conclusion: Together, our data indicate that AMPs reduced hematopoie c toxicity induced by ionizing radia on when infused within 24 h a9er radia on injury.
人羊膜源多能祖细胞对C57BL/6小鼠全身照射后造血功能恢复的影响
背景:造血系统对电离辐射的不良反应敏感。利用间充质干细胞或血管内皮细胞的细胞疗法已被探索为电离辐射治疗造血损伤的潜在对策。我们研究了从羊膜培养的门控细胞(羊膜衍生的多能干祖细胞,AMP)对小鼠全身照射后造血恢复的影响。材料和方法:C57BL/6J小鼠假照射或暴露于60 Co辐照(7.75–7.90 Gy,0.6 Gy/min)。AMPs(5×106个细胞/只)或载体经腹膜内照射24小时。结果:与载体在7.75和7.90 Gy照射后相比,AMPs处理的小鼠30天生存率分别提高了13%和20%。AMP治疗的特点是白细胞、中性粒细胞、肺细胞和单核细胞的加速恢复趋势,在第40天以7.75Gy的剂量进行测量。与载体治疗的动物相比,AMP治疗增强了脾脏和股骨髓的造血细胞再生,通过总有核细胞和造血祖细胞计数进行测量。FACS分析显示,AMPs治疗显著降低了CD11b+/Gr-1 int和CD11b+/-Gr-1高骨髓细胞群的最低点,并改善了这些细胞类型的恢复。结论:总之,我们的数据表明,在损伤后24小时内输注AMPs可降低电离辐射诱导的造血毒性。
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来源期刊
Iranian Journal of Radiation Research
Iranian Journal of Radiation Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
0.67
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Iranian Journal of Radiation Research (IJRR) publishes original scientific research and clinical investigations related to radiation oncology, radiation biology, and Medical and health physics. The clinical studies submitted for publication include experimental studies of combined modality treatment, especially chemoradiotherapy approaches, and relevant innovations in hyperthermia, brachytherapy, high LET irradiation, nuclear medicine, dosimetry, tumor imaging, radiation treatment planning, radiosensitizers, and radioprotectors. All manuscripts must pass stringent peer-review and only papers that are rated of high scientific quality are accepted.
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