Y. Du, R. Banas, Elizabeth A McCart, Jeffy George, K. Oakley, Y. Han, M. Landauer, R. Day
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引用次数: 3
Abstract
Background: The hematopoie c system is sensi ve to the adverse effects of ionizing radia on. Cellular therapies u lizing mesenchymal stem cells or vascular endothelial cells have been explored as poten al countermeasures for radia on hematopoie c injuries. We inves gated cells cultured from amnion (Amnion-derived Mul potent Progenitor cells, AMPs) for effects on hematopoie c recovery following total body irradia on in mice. Materials and Methods: C57BL/6J mice were sham-irradiated or exposed to 60 Co irradia on (7.75 – 7.90 Gy, 0.6 Gy/min). Either AMPs (5 × 10 6 cells/animal) or vehicle were administered 24 h pos rradia on via intraperitoneal injec on. Results: We observed a 13% and 20% improvement in 30-day survival of mice treated with AMPs compared with treatment with vehicle following irradia on at 7.75 and 7.90 Gy, respec vely. AMP treatment was characterized by a trend toward accelerated recovery of white blood cells, neutrophils, re culocytes, and monocytes, measured through day 40 pos rradia on a9er 7.75 Gy. AMP treatment enhanced hematopoie c cell repopula on of spleen and femoral bone marrow as measured by total nucleated cell and hematopoie c progenitor cell counts in comparison to vehicle-treated animals. FACS analysis showed that AMPs treatment significantly mi gated the reduc on in CD11b + /Gr-1 int and CD11b + /Gr-1 high bone marrow cell popula ons at the nadir, and improved recovery of these cell types. Conclusion: Together, our data indicate that AMPs reduced hematopoie c toxicity induced by ionizing radia on when infused within 24 h a9er radia on injury.
期刊介绍:
Iranian Journal of Radiation Research (IJRR) publishes original scientific research and clinical investigations related to radiation oncology, radiation biology, and Medical and health physics. The clinical studies submitted for publication include experimental studies of combined modality treatment, especially chemoradiotherapy approaches, and relevant innovations in hyperthermia, brachytherapy, high LET irradiation, nuclear medicine, dosimetry, tumor imaging, radiation treatment planning, radiosensitizers, and radioprotectors. All manuscripts must pass stringent peer-review and only papers that are rated of high scientific quality are accepted.