Bioluminescence capability and intensity in the dinoflagellate Alexandrium species

IF 3.1 3区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Algae Pub Date : 2021-12-15 DOI:10.4490/algae.2021.36.12.6

Sang Ah Park, H. Jeong, Jin Hee Ok, Hee Chang Kang, Ji Hyun You, S. Eom, Y. Yoo, Moo Joon Lee

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引用次数: 2

Abstract

Some species in the dinoflagellate genus Alexandrium are bioluminescent. Of the 33 formally described Alexandrium species, the bioluminescence capability of only nine species have been tested, and eight have been reported to be bioluminescent. The present study investigated the bioluminescence capability of seven Alexandrium species that had not been tested. Alexandrium mediterraneum, A. pohangense, and A. tamutum were bioluminescent, but A. andersonii, A. hiranoi, A. insuetum, and A. pseudogonyaulax were not. We also measured the bioluminescent intensity of A. affine, A. fraterculus, A. mediterraneum, A. ostenfeldii, A. pacificum, A. pohangense, A. tamarense, and A. tamutum. The mean 200-second-integrated bioluminescence intensity per cell ranged from 0.02 to 32.2 × 104 relative luminescence unit per cell (RLU cell-1), and the mean maximum bioluminescence intensity per cell per second (BLMax) ranged from 0.01 to 10.3 × 104 RLU cell-1 s-1-1. BLMax was significantly correlated with the maximum growth rates of Alexandrium species, except for A. tamarense. A phylogenetic tree based on large subunit ribosomal DNA (LSU rDNA) showed that the bioluminescent species A. affine, A. catenella, A. fraterculus, A. mediterraneum, A. pacificum, and A. tamarense formed a large clade. However, the toxicity or mixotrophic capability of these species was split. Thus, their bioluminescence capability in this clade was more consistent than their toxicity or mixotrophic capability. Phylogenetic trees based on LSU rDNA and the luciferase gene of Alexandrium were consistent except for A. pohangense. The results of the present study can provide a basis for understanding the interspecific diversity in bioluminescence of Alexandrium.

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亚历山大藻的生物发光能力和强度

亚历山大藻属甲藻中的一些物种具有生物发光性。在33个正式描述的亚历山大藻物种中，只有9个物种的生物发光能力得到了测试，据报道有8个是生物发光的。本研究调查了七种未经测试的亚历山大藻的生物发光能力。地中海亚历山大藻、波汉根塞亚历山大藻和塔穆图姆亚历山大藻是生物发光的，而安氏亚历山大藻、平氏亚历山大藻和假生殖亚历山大藻则不是。我们还测量了A.affine、A.fraterculus、A.mediteraneum、A.ostnfeldii、A.pacificum、A.pohangense、A.tamarense和A.tamutum的生物发光强度。每个细胞200秒的平均积分生物发光强度为0.02至32.2×104相对发光单位/细胞（RLU cell-1），每个细胞每秒的平均最大生物发光强度（BLMax）为0.01至10.3×104 RLU cell-1s-1-1。BLMax与亚历山大藻（A.tamarense除外）的最大生长速率显著相关。基于大亚基核糖体DNA（LSU rDNA）的系统发育树表明，生物发光物种A.affine、A.catenella、A.fraterculus、A.mediteraneum、A.pacificum和A.tamarense形成了一个大的分支。然而，这些物种的毒性或混合营养能力是分裂的。因此，它们在该分支中的生物发光能力比它们的毒性或混合营养能力更一致。基于LSU rDNA和亚历山大藻萤光素酶基因的系统发育树除A.pohangense外基本一致。本研究结果可为了解亚历山大藻生物发光的种间多样性提供依据。

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来源期刊

Algae PLANT SCIENCES-

CiteScore

5.10

自引率

25.00%

发文量

期刊介绍： ALGAE is published by the Korean Society of Phycology and provides prompt publication of original works on phycology. ALGAE publishes articles on all aspects of phylogenetics and taxonomy, ecology and population biology, physiology and biochemistry, cell and molecular biology, and biotechnology and applied phycology. Checklists or equivalent manu-scripts may be considered for publication only if they contribute original information on taxonomy (e.g., new combinations), ecology or biogeography of more than just local relevance. Contributions may take the form of Original Research Articles, Research Notes, Review Articles and Book Reviews.